B cell epitope mapping using synthetic peptides - PubMed

Review

B cell epitope mapping using synthetic peptides

John Mark Carter et al. Curr Protoc Immunol. 2004 May.

Abstract

Synthetic peptides are used for identification of functional B cell epitopes in antibody preparations. ELISA-type assays are used to identify sequences of proteins comprising antibody-binding regions. This unit describes three peptide display formats. Pepscan (pins) and SPOTs (cellulose membranes) may be used as solid-phase support media for peptide synthesis, followed by ELISA directly on the resulting peptide array. Alternatively, peptides may be cleaved from the array and tested in a standard microplate-based antibody capture ELISA format. The discussion includes choosing the peptide sequences by length and overlap, as well as determination of the minimum essential sequence for antibody binding. This method is highly effective for continuous epitopes and is often also useful for discontinuous epitopes.

Similar articles

• Screening and Characterization of Linear B-Cell Epitopes by Biotinylated Peptide Libraries.

  Rosenkrands I, Olsen A. Rosenkrands I, et al. Methods Mol Biol. 2015;1348:241-50. doi: 10.1007/978-1-4939-2999-3_21. Methods Mol Biol. 2015. PMID: 26424277

• Synthetic Peptide-Based ELISA and ELISpot Assay for Identifying Autoantibody Epitopes.

  Pozsgay J, Szarka E, Huber K, Babos F, Magyar A, Hudecz F, Sarmay G. Pozsgay J, et al. Methods Mol Biol. 2016;1352:223-33. doi: 10.1007/978-1-4939-3037-1_17. Methods Mol Biol. 2016. PMID: 26490479

• B-Cell Epitope Mapping Using a Library of Overlapping Synthetic Peptides in an Enzyme-Linked Immunosorbent Assay.

  Vanniasinkam T, Barton MD, Das TP, Heuzenroeder MW. Vanniasinkam T, et al. Methods Mol Biol. 2018;1785:121-128. doi: 10.1007/978-1-4939-7841-0_8. Methods Mol Biol. 2018. PMID: 29714015

• B cell epitope mapping: The journey to better vaccines and therapeutic antibodies.

  De Leon AJ, Tjiam MC, Yu Y. De Leon AJ, et al. Biochim Biophys Acta Gen Subj. 2024 Oct;1868(10):130674. doi: 10.1016/j.bbagen.2024.130674. Epub 2024 Jul 28. Biochim Biophys Acta Gen Subj. 2024. PMID: 39079649 Review.

• In silico models for B-cell epitope recognition and signaling.

  Ansari HR, Raghava GP. Ansari HR, et al. Methods Mol Biol. 2013;993:129-38. doi: 10.1007/978-1-62703-342-8_9. Methods Mol Biol. 2013. PMID: 23568468 Review.

Cited by

• Identification of a highly conserved H1 subtype-specific epitope with diagnostic potential in the hemagglutinin protein of influenza A virus.

  Zhao R, Cui S, Guo L, Wu C, Gonzalez R, Paranhos-Baccalà G, Vernet G, Wang J, Hung T. Zhao R, et al. PLoS One. 2011;6(8):e23374. doi: 10.1371/journal.pone.0023374. Epub 2011 Aug 19. PLoS One. 2011. PMID: 21886787 Free PMC article.

• Characterizing affinity epitopes between prion protein and β-amyloid using an epitope mapping immunoassay.

  Kang M, Kim SY, An SS, Ju YR. Kang M, et al. Exp Mol Med. 2013 Aug 2;45(8):e34. doi: 10.1038/emm.2013.63. Exp Mol Med. 2013. PMID: 23907583 Free PMC article.

• Misfolded proteins induce aggregation of the lectin Yos9.

  Smith MH, Rodriguez EH, Weissman JS. Smith MH, et al. J Biol Chem. 2014 Sep 12;289(37):25670-7. doi: 10.1074/jbc.M114.583344. Epub 2014 Aug 1. J Biol Chem. 2014. PMID: 25086047 Free PMC article.

• Progress in the Analysis of Food Allergens through Molecular Biology Approaches.

  Volpicella M, Leoni C, Dileo MCG, Ceci LR. Volpicella M, et al. Cells. 2019 Sep 12;8(9):1073. doi: 10.3390/cells8091073. Cells. 2019. PMID: 31547388 Free PMC article. Review.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Wiley

• Other Literature Sources

  • The Lens - Patent Citations Database