Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material - PubMed

Affiliations

• PMID: 1867860

Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material

P S Walsh et al. Biotechniques. 1991 Apr.

Abstract

Procedures utilizing Chelex 100 chelating resin have been developed for extracting DNA from forensic-type samples for use with the PCR. The procedures are simple, rapid, involve no organic solvents and do not require multiple tube transfers for most types of samples. The extraction of DNA from semen and very small bloodstains using Chelex 100 is as efficient or more efficient than using proteinase K and phenol-chloroform extraction. DNA extracted from bloodstains seems less prone to contain PCR inhibitors when prepared by this method. The Chelex method has been used with amplification and typing at the HLA DQ alpha locus to obtain the DQ alpha genotypes of many different types of samples, including whole blood, bloodstains, seminal stains, buccal swabs, hair and post-coital samples. The results of a concordance study are presented in which the DQ alpha genotypes of 84 samples prepared using Chelex or using conventional phenol-chloroform extraction are compared. The genotypes obtained using the two different extraction methods were identical for all samples tested.

Similar articles

• Recovery of DNA for PCR amplification from blood and forensic samples using a chelating resin.

  Willard JM, Lee DA, Holland MM. Willard JM, et al. Methods Mol Biol. 1998;98:9-18. doi: 10.1385/0-89603-443-7:9. Methods Mol Biol. 1998. PMID: 9664549 No abstract available.

• Report of the blind trial of the Cetus Amplitype HLA DQ alpha forensic deoxyribonucleic acid (DNA) amplification and typing kit.

  Walsh PS, Fildes N, Louie AS, Higuchi R. Walsh PS, et al. J Forensic Sci. 1991 Sep;36(5):1551-6. J Forensic Sci. 1991. PMID: 1955839

• PCR amplification and typing of the HLA DQ alpha gene in forensic samples.

  Comey CT, Budowle B, Adams DE, Baumstark AL, Lindsey JA, Presley LA. Comey CT, et al. J Forensic Sci. 1993 Mar;38(2):239-49. J Forensic Sci. 1993. PMID: 8095967

• Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. BioTechniques 10(4): 506-13 (April 1991).

  Walsh PS, Metzger DA, Higushi R. Walsh PS, et al. Biotechniques. 2013 Mar;54(3):134-9. doi: 10.2144/000114018. Biotechniques. 2013. PMID: 23599926 No abstract available.

• DNA research in forensic dentistry.

  Lijnen I, Willems G. Lijnen I, et al. Methods Find Exp Clin Pharmacol. 2001 Nov;23(9):511-7. doi: 10.1358/mf.2001.23.9.662139. Methods Find Exp Clin Pharmacol. 2001. PMID: 11876026 Review.

Cited by

• Development and population study of the 12 X-STR loci multiplexes PCR systems.

  Liu QL, Zhao H, Chen JD, Wang XG, Lu DJ, Quan L. Liu QL, et al. Int J Legal Med. 2012 Jul;126(4):665-70. doi: 10.1007/s00414-012-0676-8. Epub 2012 Feb 16. Int J Legal Med. 2012. PMID: 22349757

• Reconstruction of a windborne insect invasion using a particle dispersal model, historical wind data, and Bayesian analysis of genetic data.

  Lander TA, Klein EK, Oddou-Muratorio S, Candau JN, Gidoin C, Chalon A, Roig A, Fallour D, Auger-Rozenberg MA, Boivin T. Lander TA, et al. Ecol Evol. 2014 Dec;4(24):4609-25. doi: 10.1002/ece3.1206. Epub 2014 Dec 2. Ecol Evol. 2014. PMID: 25558356 Free PMC article.

• Facultative asexual reproduction and genetic diversity of populations in the humivorous termite Cavitermes tuberosus.

  Fournier D, Hellemans S, Hanus R, Roisin Y. Fournier D, et al. Proc Biol Sci. 2016 Jun 15;283(1832):20160196. doi: 10.1098/rspb.2016.0196. Proc Biol Sci. 2016. PMID: 27252019 Free PMC article.

• Contact-free isolation of sperm and epithelial cells by laser microdissection and pressure catapulting.

  Seidl S, Burgemeister R, Hausmann R, Betz P, Lederer T. Seidl S, et al. Forensic Sci Med Pathol. 2005 Jun;1(2):153-7. doi: 10.1385/FSMP:1:2:153. Forensic Sci Med Pathol. 2005. PMID: 25869953

• Vibrio Species in an Urban Tropical Estuary: Antimicrobial Susceptibility, Interaction with Environmental Parameters, and Possible Public Health Outcomes.

  Canellas ALB, Lopes IR, Mello MP, Paranhos R, de Oliveira BFR, Laport MS. Canellas ALB, et al. Microorganisms. 2021 May 7;9(5):1007. doi: 10.3390/microorganisms9051007. Microorganisms. 2021. PMID: 34067081 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Other Literature Sources

  • The Lens - Patent Citations Database

• Research Materials

  • NCI CPTC Antibody Characterization Program