A murine monoclonal antibody that binds N-terminal extracellular segment of human protease-activated receptor-4 - PubMed

A murine monoclonal antibody that binds N-terminal extracellular segment of human protease-activated receptor-4

Takeshi Sangawa et al. Hybridoma (Larchmt). 2008 Oct.

Abstract

Abstract A monoclonal antibody that recognizes native G protein coupled receptors (GPCR) is generally difficult to obtain. Protease-activated receptor-4 (PAR4) is a GPCR that plays an important role in platelet activation as a low-affinity thrombin receptor. By immunizing peptide corresponding to the N-terminal segment of human PAR4, we obtained a monoclonal antibody that recognizes cell surface expressed PAR4. Epitope mapping using a series of artificial fusion proteins that carry PAR4-derived peptide revealed that the recognition motif is fully contained within the 6-residue portion adjacent to the thrombin cleavage site. The antibody blocked PAR4 peptide cleavage by thrombin, suggesting its utility in the functional study of PAR4 signaling.

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