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Overcoming the solubility limit with solubility-enhancement tags: successful applications in biomolecular NMR studies - PubMed

Overcoming the solubility limit with solubility-enhancement tags: successful applications in biomolecular NMR studies

Pei Zhou et al. J Biomol NMR. 2010 Jan.

Abstract

Although the rapid progress of NMR technology has significantly expanded the range of NMR-trackable systems, preparation of NMR-suitable samples that are highly soluble and stable remains a bottleneck for studies of many biological systems. The application of solubility-enhancement tags (SETs) has been highly effective in overcoming solubility and sample stability issues and has enabled structural studies of important biological systems previously deemed unapproachable by solution NMR techniques. In this review, we provide a brief survey of the development and successful applications of the SET strategy in biomolecular NMR.We also comment on the criteria for choosing optimal SETs, such as for differently charged target proteins, and recent new developments on NMR-invisible SETs.

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Figures

**Figure 1**
HSQC spectra of ¹⁵N-labeled DFF45 N-terminal CIDE Domain in complex with unlabeled DFF40 (1–80). Attachment of the GB1 tag significantly increased the solubility and stability of the DFF40/45 complex and generated superior NMR spectra. Arrows indicate distinct resonances from DFF45 in the DFF40/45 complex. (Reprinted with permission from Figure 1bc of (Zhou et al. 2001b), *Journal of Biomolecular NMR*)

**Figure 2**
NMR-invisible solubility-enhancement tags.

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Overcoming the solubility limit with solubility-enhancement tags: successful applications in biomolecular NMR studies - PubMed