pubmed.ncbi.nlm.nih.gov

14-3-3 isoforms participate in red light signaling and photoperiodic flowering - PubMed

14-3-3 isoforms participate in red light signaling and photoperiodic flowering

Kevin M Folta et al. Plant Signal Behav. 2008 May.

Abstract

Members of the 14-3-3 family of proteins participate in signal transduction by modulating flux through various pathways. Potential subfunctionalization within this family has produced a suite of related proteins with diverse client interactions and discrete localization. The associated study assesses the biological roles of two specific 14-3-3 isoforms, using genetic, biochemical and physiological assays to ascertain potential nodes of interaction. Arabidopsis T-DNA insertion mutants representing the nu and mu isoforms exhibited a short, yet clear delay in flowering time on long days. Tests of hypocotyl growth inhibition under narrow bandwidth light indicated a hyposensitivity to red light, while responses to blue and far-red light were normal. These physiological tests suggest a mechanistic link between 14-3-3 proteins, red light sensing, and the pathways that control photoperiodic flowering. The precise entry point into the pathway was assessed using yeast two hybrid assays targeted against specific proteins active in the circadian oscillator, light transduction and photoperiodic flowering. Yeast two hybrid interaction was observed with CONSTANS (CO), and then confirmed with coimmunoprecipitation. Functional interaction with phyB leading to defects in flowering time and direct interaction with CONSTANS circumstantially places these specific 14-3-3 isoforms into the pathway that regulates the transition between vegetative and floral development.

Keywords: isoform specificity; phosphorylation; protein interaction; signaling.

PubMed Disclaimer

Comment on

Similar articles

Cited by

References

    1. van Heusden GP, van der Zanden AL, Ferl RJ, Steensma HY. Four Arabidopsis thaliana 14-3-3 protein isoforms can complement the lethal yeast bmh1 bmh2 double disruption. FEBS Lett. 1996;391:252–256. - PubMed
    1. Lu G, de Vetten NC, Sehnke PC, Isobe T, Ichimura T, Fu H, van Heusden GP, Ferl RJ. A single Arabidopsis GF14 isoform possesses biochemical characteristics of diverse 14-3-3 homologues. Plant Mol Biol. 1994;25:659–667. - PubMed
    1. Paul AL, Sehnke PC, Ferl RJ. Isoform-specific subcellular localization among 14-3-3 proteins in Arabidopsis seems to be driven by client interactions. Mol Biol Cell. 2005;16:1735–1743. - PMC - PubMed
    1. Ferl RJ. 14-3-3 proteins: Regulation of signal-induced events. Physiol Plant. 2004;120:173–178. - PubMed
    1. Sehnke PC, DeLille JM, Ferl RJ. Consummating signal transduction: The role of 14-3-3 proteins in the completion of signal-induced transitions in protein activity. Plant Cell. 2002;14:S339–S354. - PMC - PubMed

LinkOut - more resources