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Development of immunoglobulin M memory to both a T-cell-independent and a T-cell-dependent antigen following infection with Vibrio cholerae O1 in Bangladesh - PubMed

Development of immunoglobulin M memory to both a T-cell-independent and a T-cell-dependent antigen following infection with Vibrio cholerae O1 in Bangladesh

Emily A Kendall et al. Infect Immun. 2010 Jan.

Abstract

Vibrio cholerae O1 can cause severe watery diarrhea that can be life-threatening without treatment. Infection results in long-lasting protection against subsequent disease. Development of memory B cells of the immunoglobulin G (IgG) and IgA isotypes to V. cholerae O1 antigens, including serotype-specific lipopolysaccharide (LPS) and the B subunit of cholera toxin (CTB), after cholera infection has been demonstrated. Memory B cells of the IgM isotype may play a role in long-term protection, particularly against T-cell-independent antigens, but IgM memory has not been studied in V. cholerae O1 infection. Therefore, we assayed acute- and convalescent-phase blood samples from cholera patients for the presence of memory B cells that produce cholera antigen-specific IgM antibody upon polyclonal stimulation in in vitro culture. We also examined the development of serological and antibody-secreting cell responses following infection. Subjects developed significant IgM memory responses by day 30 after infection, both to the T-cell-independent antigen LPS and to the T-cell-dependent antigen CTB. No significant corresponding elevations in plasma IgM antibodies or circulating IgM antibody-secreting cells to CTB were detected. In 17 subjects followed to day 90 after infection, significant persistence of elevated IgM memory responses was not observed. The IgM memory response to CTB was negatively correlated with the IgG plasma antibody response to CTB, and there was a trend toward negative correlation between the IgM memory and IgA plasma antibody responses to LPS. We did not observe an association between the IgM memory response to LPS and the vibriocidal titer.

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Figures

FIG. 1.
FIG. 1.

IgA, IgG, and IgM serological responses to CTB and LPS antigens in cholera patients at near baseline (day 2 after onset of illness), acute stage (day 7), and convalescence (day 30). *, Significant elevation (P < 0.002) in comparison to day 2.

FIG. 2.
FIG. 2.

Antigen-specific circulating ASCs of IgA, IgG, and IgM isotypes in cholera patients 2, 7, and 30 days after onset of illness. *, P < 0.05 in comparison to day 2; n = 15, six of whom were included in other analyses.

FIG. 3.
FIG. 3.

Antigen-specific B-cell memory responses of IgA, IgG, and IgM isotypes in cholera patients 2, 30, and 90 days after onset of illness, determined from ELISA measurements of lymphocyte culture supernatant specimens. *, P < 0.05 in comparison to day 2.

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References

    1. Ahmed, T., A. M. Svennerholm, A. Al Tarique, G. N. Sultana, and F. Qadri. 2009. Enhanced immunogenicity of an oral inactivated cholera vaccine in infants in Bangladesh obtained by zinc supplementation and by temporary withholding breast-feeding. Vaccine 27:1433-1439. - PubMed
    1. Bjornson, A. B., and P. A. Detmers. 1995. The pentameric structure of IgM is necessary to enhance opsonization of Bacteroides thetaiotaomicron and Bacteroides fragilis via the alternative complement pathway. Microb. Pathog. 19:117-128. - PubMed
    1. Capolunghi, F., S. Cascioli, E. Giorda, M. M. Rosado, A. Plebani, C. Auriti, G. Seganti, R. Zuntini, S. Ferrari, M. Cagliuso, I. Quinti, and R. Carsetti. 2008. CpG drives human transitional B cells to terminal differentiation and production of natural antibodies. J. Immunol. 180:800-808. - PubMed
    1. Clements, M. L., M. M. Levine, C. R. Young, R. E. Black, Y. L. Lim, R. M. Robins-Browne, and J. P. Craig. 1982. Magnitude, kinetics, and duration of vibriocidal antibody responses in North Americans after ingestion of Vibrio cholerae. J. Infect. Dis. 145:465-473. - PubMed
    1. Crotty, S., R. D. Aubert, J. Glidewell, and R. Ahmed. 2004. Tracking human antigen-specific memory B cells: a sensitive and generalized ELISPOT system. J. Immunol. Methods 286:111-122. - PubMed

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