Erythroid differentiation in chimaeric mice blocked by a targeted mutation in the gene for transcription factor GATA-1 - PubMed

Affiliations

• PMID: 1987478
• DOI: 10.1038/349257a0

Erythroid differentiation in chimaeric mice blocked by a targeted mutation in the gene for transcription factor GATA-1

L Pevny et al. Nature. 1991.

Abstract

The zinc-finger transcription factor GATA-1 (previously known as GF-1, NF-E1 or Eryf 1 binds to GATA consensus elements in regulatory regions of the alpha- and beta-globin gene clusters and other erythroid cell-specific genes. Analysis of the effects of mutations in GATA-binding sites in cell culture and in binding assays in vitro, as well as transactivation studies with GATA-1 expression vectors in heterologous cells, have provided indirect evidence that this factor is involved in the activation of globin and other genes during erythroid cell maturation. GATA-1 is also expressed in megakaryocytes and mast cells, but not in other blood cell lineages or in non-haemopoietic cells. To investigate the role of this factor in haematopoiesis in vivo, we disrupted the X-linked GATA-1 gene by homologous recombination in a male (XY) murine embryonic stem cell line and tested the GATA-1-deficient cells for their ability to contribute to different tissues in chimaeric mice. The mutant embryonic stem cells contributed to all non-haemopoietic tissues tested and to a white blood cell fraction, but failed to give rise to mature red blood cells. This demonstrates that GATA-1 is required for the normal differentiation of erythroid cells, and that other GATA-binding proteins cannot compensate for its absence.

Similar articles

• Knock-in mutation of transcription factor GATA-3 into the GATA-1 locus: partial rescue of GATA-1 loss of function in erythroid cells.

  Tsai FY, Browne CP, Orkin SH. Tsai FY, et al. Dev Biol. 1998 Apr 15;196(2):218-27. doi: 10.1006/dbio.1997.8842. Dev Biol. 1998. PMID: 9576834

• Transgenic over-expression of GATA-1 mutant lacking N-finger domain causes hemolytic syndrome in mouse erythroid cells.

  Nakano M, Ohneda K, Yamamoto-Mukai H, Shimizu R, Ohneda O, Ohmura S, Suzuki M, Tsukamoto S, Yanagawa T, Yoshida H, Takakuwa Y, Yamamoto M. Nakano M, et al. Genes Cells. 2005 Jan;10(1):47-62. doi: 10.1111/j.1365-2443.2005.00814.x. Genes Cells. 2005. PMID: 15670213

• An intrinsic but cell-nonautonomous defect in GATA-1-overexpressing mouse erythroid cells.

  Whyatt D, Lindeboom F, Karis A, Ferreira R, Milot E, Hendriks R, de Bruijn M, Langeveld A, Gribnau J, Grosveld F, Philipsen S. Whyatt D, et al. Nature. 2000 Aug 3;406(6795):519-24. doi: 10.1038/35020086. Nature. 2000. PMID: 10952313

• Rescue of erythroid development in gene targeted GATA-1- mouse embryonic stem cells.

  Simon MC, Pevny L, Wiles MV, Keller G, Costantini F, Orkin SH. Simon MC, et al. Nat Genet. 1992 May;1(2):92-8. doi: 10.1038/ng0592-92. Nat Genet. 1992. PMID: 1302015 Review.

• GATA-1: friends, brothers, and coworkers.

  Morceau F, Schnekenburger M, Dicato M, Diederich M. Morceau F, et al. Ann N Y Acad Sci. 2004 Dec;1030:537-54. doi: 10.1196/annals.1329.064. Ann N Y Acad Sci. 2004. PMID: 15659837 Review.

Cited by

• The absence of eosinophils is associated with early metastatic lesions in Leishmania amazonensis-infected mice.

  Almeida GG, Luehring TAM, Paixão PHM, Soares RP, de Barros ALB, do Monte-Neto RL, Tafuri WL, Negrão-Corrêa DA, Gonçalves R. Almeida GG, et al. Mem Inst Oswaldo Cruz. 2024 Jan 8;119:e220242. doi: 10.1590/0074-02760220242. eCollection 2024. Mem Inst Oswaldo Cruz. 2024. PMID: 38198296 Free PMC article.

• Spectrum of myeloid neoplasms and immune deficiency associated with germline GATA2 mutations.

  Mir MA, Kochuparambil ST, Abraham RS, Rodriguez V, Howard M, Hsu AP, Jackson AE, Holland SM, Patnaik MM. Mir MA, et al. Cancer Med. 2015 Apr;4(4):490-9. doi: 10.1002/cam4.384. Epub 2015 Jan 26. Cancer Med. 2015. PMID: 25619630 Free PMC article. Review.

• Cell-specific transcription of the peripherin gene in neuronal cell lines involves a cis-acting element surrounding the TATA box.

  Desmarais D, Filion M, Lapointe L, Royal A. Desmarais D, et al. EMBO J. 1992 Aug;11(8):2971-80. doi: 10.1002/j.1460-2075.1992.tb05367.x. EMBO J. 1992. PMID: 1639068 Free PMC article.

• A mouse oocyte-specific protein that binds to a region of mZP3 promoter responsible for oocyte-specific mZP3 gene expression.

  Schickler M, Lira SA, Kinloch RA, Wassarman PM. Schickler M, et al. Mol Cell Biol. 1992 Jan;12(1):120-7. doi: 10.1128/mcb.12.1.120-127.1992. Mol Cell Biol. 1992. PMID: 1729594 Free PMC article.

• GATA switches as developmental drivers.

  Bresnick EH, Lee HY, Fujiwara T, Johnson KD, Keles S. Bresnick EH, et al. J Biol Chem. 2010 Oct 8;285(41):31087-93. doi: 10.1074/jbc.R110.159079. Epub 2010 Jul 29. J Biol Chem. 2010. PMID: 20670937 Free PMC article. Review.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Nature Publishing Group

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • BioCyc
  • Gene Ontology
  • Mouse Genome Informatics (MGI)

• Research Materials

  • Cellosaurus - a cell line knowledge resource