NMR investigation of DNA primer-template models: guanine templates are less prone to strand slippage upon misincorporation - PubMed

Affiliations

• PMID: 19886640
• DOI: 10.1021/bi9014049

NMR investigation of DNA primer-template models: guanine templates are less prone to strand slippage upon misincorporation

Lai Man Chi et al. Biochemistry. 2009.

Abstract

Misaligned structures can result from strand slippage during DNA replication and, if not repaired, would lead to mutations. Previously, we showed that strand slippage can occur upon misincorporation of a dNTP opposite thymine and cytosine templates, resulting in a misaligned structure with a T- or C-bulge. The formation propensity for misaligned structures was found to depend on the type of terminal base pair. In this study, we performed NMR investigations on primer-template models containing a guanine template. Our results reveal guanine templates are less prone to strand slippage than pyrimidine templates. Misalignment was found to occur only in 5'-CG templates with a downstream purine. In addition to the significance of terminal base pair and upstream nucleotide, the present study reveals the importance of the templating base and its downstream nucleotide, which also determine the propensity of strand slippage in primer-templates.

Similar articles

• Sequence context effect on strand slippage in natural DNA primer-templates.

  Chi LM, Lam SL. Chi LM, et al. J Phys Chem B. 2012 Feb 16;116(6):1999-2007. doi: 10.1021/jp211666k. Epub 2012 Feb 3. J Phys Chem B. 2012. PMID: 22304666

• NMR investigation of primer-template models: structural effect of sequence downstream of a thymine template on mutagenesis in DNA replication.

  Chi LM, Lam SL. Chi LM, et al. Biochemistry. 2007 Aug 14;46(32):9292-300. doi: 10.1021/bi700865e. Epub 2007 Jul 21. Biochemistry. 2007. PMID: 17658896

• Nuclear magnetic resonance investigation of primer--template models: formation of a pyrimidine bulge upon misincorporation.

  Chi LM, Lam SL. Chi LM, et al. Biochemistry. 2008 Apr 15;47(15):4469-76. doi: 10.1021/bi8001456. Epub 2008 Mar 22. Biochemistry. 2008. PMID: 18358004

• Encoded errors: mutations and rearrangements mediated by misalignment at repetitive DNA sequences.

  Lovett ST. Lovett ST. Mol Microbiol. 2004 Jun;52(5):1243-53. doi: 10.1111/j.1365-2958.2004.04076.x. Mol Microbiol. 2004. PMID: 15165229 Review.

• NMR structures of damaged DNA.

  Lukin M, de Los Santos C. Lukin M, et al. Chem Rev. 2006 Feb;106(2):607-86. doi: 10.1021/cr0404646. Chem Rev. 2006. PMID: 16464019 Review. No abstract available.

Cited by

• On the sequence-directed nature of human gene mutation: the role of genomic architecture and the local DNA sequence environment in mediating gene mutations underlying human inherited disease.

  Cooper DN, Bacolla A, Férec C, Vasquez KM, Kehrer-Sawatzki H, Chen JM. Cooper DN, et al. Hum Mutat. 2011 Oct;32(10):1075-99. doi: 10.1002/humu.21557. Epub 2011 Sep 2. Hum Mutat. 2011. PMID: 21853507 Free PMC article. Review.

• Cytosine unstacking and strand slippage at an insertion-deletion mutation sequence in an overhang-containing DNA duplex.

  Manjari SR, Pata JD, Banavali NK. Manjari SR, et al. Biochemistry. 2014 Jun 17;53(23):3807-16. doi: 10.1021/bi500189g. Epub 2014 Jun 9. Biochemistry. 2014. PMID: 24854722 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • American Chemical Society