Catabolism of adenine nucleotides in suspension-cultured plant cells - PubMed

Catabolism of adenine nucleotides in suspension-cultured plant cells

N Yabuki et al. Biochim Biophys Acta. 1991.

Abstract

Profiles of the catabolism of adenine nucleotides in cultured plant cells were investigated. Adenine nucleotides, prelabelled by incubation of suspension-cultured Catharantus roseus cells with [8-14C]adenosine, were catabolized rapidly and most of the radioactivity appeared in 14CO2. Allantoin and allantoic acid, intermediates of the oxidative catabolic pathway of purines, were temporarily labelled. When the cells, prelabelled with [8-14C]adenosine, were incubated with high concentrations of adenosine, the rate of catabolism of adenine nucleotides increased. The results suggest that the relative rate of catabolism of adenine nucleotides is strongly dependent on the concentration of adenine nucleotides in the cells. Studies using allopurinol, coformycin and tiazofurin, inhibitors of enzymes involved in purine metabolism, suggest that participation of AMP deaminase and xanthine oxidoreductase in the catabolism of adenine nucleotides in plant cells. AMP deaminase was found in extracts from C. roseus cells and its activity increased significantly in the presence of ATP. In contrast, no adenosine deaminase or adenine deaminase activity was detected. Qualitative differences in the catabolic activity of AMP were observed between suspension-cultured cells from different species of plants.

Similar articles

• Purine catabolism in isolated rat hepatocytes. Influence of coformycin.

  Van den Berghe G, Bontemps F, Hers HG. Van den Berghe G, et al. Biochem J. 1980 Jun 15;188(3):913-20. doi: 10.1042/bj1880913. Biochem J. 1980. PMID: 7470045 Free PMC article.

• Pathways of adenine nucleotide catabolism in primary rat muscle cultures.

  Zoref-Shani E, Shainberg A, Sperling O. Zoref-Shani E, et al. Biochim Biophys Acta. 1987 Dec 7;926(3):287-95. doi: 10.1016/0304-4165(87)90215-7. Biochim Biophys Acta. 1987. PMID: 2825800

• Fructose-induced adenine nucleotide catabolism in isolated rat hepatocytes.

  Smith CM, Rovamo LM, Raivio KO. Smith CM, et al. Can J Biochem. 1977 Dec;55(12):1237-40. doi: 10.1139/o77-185. Can J Biochem. 1977. PMID: 597772

• Enzymes involved in purine metabolism--a review of histochemical localization and functional implications.

  Moriwaki Y, Yamamoto T, Higashino K. Moriwaki Y, et al. Histol Histopathol. 1999 Oct;14(4):1321-40. doi: 10.14670/HH-14.1321. Histol Histopathol. 1999. PMID: 10506947 Review.

• Aminohydrolases acting on adenine, adenosine and their derivatives.

  Pospisilova H, Frebort I. Pospisilova H, et al. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2007 Jun;151(1):3-10. doi: 10.5507/bp.2007.001. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2007. PMID: 17690732 Review.

Cited by

• Xanthine dehydrogenase and aldehyde oxidase impact plant hormone homeostasis and affect fruit size in 'Hass' avocado.

  Taylor NJ, Cowan AK. Taylor NJ, et al. J Plant Res. 2004 Apr;117(2):121-30. doi: 10.1007/s10265-003-0136-0. Epub 2004 Mar 10. J Plant Res. 2004. PMID: 15015080

• Adenine nucleotide pool perturbation is a metabolic trigger for AMP deaminase inhibitor-based herbicide toxicity.

  Sabina RL, Paul AL, Ferl RJ, Laber B, Lindell SD. Sabina RL, et al. Plant Physiol. 2007 Apr;143(4):1752-60. doi: 10.1104/pp.107.096487. Epub 2007 Feb 16. Plant Physiol. 2007. PMID: 17307896 Free PMC article.

• Enhanced nucleotide analysis enables the quantification of deoxynucleotides in plants and algae revealing connections between nucleoside and deoxynucleoside metabolism.

  Straube H, Niehaus M, Zwittian S, Witte CP, Herde M. Straube H, et al. Plant Cell. 2021 Apr 17;33(2):270-289. doi: 10.1093/plcell/koaa028. Plant Cell. 2021. PMID: 33793855 Free PMC article.

• Adenine aminohydrolase from Leishmania donovani: unique enzyme in parasite purine metabolism.

  Boitz JM, Strasser R, Hartman CU, Jardim A, Ullman B. Boitz JM, et al. J Biol Chem. 2012 Mar 2;287(10):7626-39. doi: 10.1074/jbc.M111.307884. Epub 2012 Jan 11. J Biol Chem. 2012. PMID: 22238346 Free PMC article.

• Purine and pyrimidine nucleotide synthesis and metabolism.

  Moffatt BA, Ashihara H. Moffatt BA, et al. Arabidopsis Book. 2002;1:e0018. doi: 10.1199/tab.0018. Epub 2002 Apr 4. Arabidopsis Book. 2002. PMID: 22303196 Free PMC article. No abstract available.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Other Literature Sources

  • The Lens - Patent Citations Database

• Research Materials

  • NCI CPTC Antibody Characterization Program