Screen of Bacillus thuringiensis toxins for transgenic rice to control Sesamia inferens and Chilo suppressalis - PubMed

Screen of Bacillus thuringiensis toxins for transgenic rice to control Sesamia inferens and Chilo suppressalis

Yulin Gao et al. J Invertebr Pathol. 2010 Sep.

Abstract

Transgenic rice to control stem borer damage is under development in China. To assess the potential of Bacillus thuringiensis (Bt) transgenes in stem borer control, the toxicity of five Bt protoxins (Cry1Aa, Cry1Ab, Cry1Ac, Cry1Ba and Cry1Ca) against two rice stem borers, Sesamia inferens (pink stem borer) and Chilo suppressalis (striped stem borer), was evaluated in the laboratory by feeding neonate larvae on artificial diets containing Bt protoxins. The results indicated that Cry1Ca exhibited the highest level of toxicity to both stem borers, with an LC(50) of 0.24 and 0.30 microg/g for C. suppressalis and S. inferens, respectively. However, S. inferens was 4-fold lower in susceptibility to Cry1Aa, and 6- and 47-fold less susceptible to Cry1Ab and Cry1Ba, respectively, compared to C. suppressalis. To evaluate interactions among Bt protoxins in stem borer larvae, toxicity assays were performed with mixtures of Cry1Aa/Cry1Ab, Cry1Aa/Cry1Ca, Cry1Ac/Cry1Ca, Cry1Ac/Cry1Ba, Cry1Ab/Cry1Ac, Cry1Ab/Cry1Ba, and Cry1Ab/Cry1Ca at 1:1 (w/w) ratios. All protoxin mixtures demonstrated significant synergistic toxicity activity against C. suppressalis, with values of 1.6- to 11-fold higher toxicity than the theoretical additive effect. Surprisingly, all but one of the Bt protoxin mixtures were antagonistic in toxicity to S. inferens. In mortality-time response experiments, S. inferens demonstrated increased tolerance to Cry1Ab and Cry1Ac compared to C. suppressalis when treated with low or high protoxin concentrations. The data indicate the utility of Cry1Ca protoxin and a Cry1Ac/Cry1Ca mixture to control both stem borer populations.

Similar articles

• Binding site concentration explains the differential susceptibility of Chilo suppressalis and Sesamia inferens to Cry1A-producing rice.

  Han L, Han C, Liu Z, Chen F, Jurat-Fuentes JL, Hou M, Peng Y. Han L, et al. Appl Environ Microbiol. 2014 Aug;80(16):5134-40. doi: 10.1128/AEM.01544-14. Epub 2014 Jun 13. Appl Environ Microbiol. 2014. PMID: 24928872 Free PMC article.

• Toxicity of Bacillus thuringiensis delta-endotoxins against bean shoot borer (Epinotia aporema Wals.) larvae, a major soybean pest in Argentina.

  Sauka DH, Sánchez J, Bravo A, Benintende GB. Sauka DH, et al. J Invertebr Pathol. 2007 Feb;94(2):125-9. doi: 10.1016/j.jip.2006.09.002. Epub 2006 Oct 27. J Invertebr Pathol. 2007. PMID: 17069845

• Susceptibility of legume pod borer (LPB), Maruca vitrata to delta-endotoxins of Bacillus thuringiensis (Bt) in Taiwan.

  Srinivasan R. Srinivasan R. J Invertebr Pathol. 2008 Jan;97(1):79-81. doi: 10.1016/j.jip.2007.06.005. Epub 2007 Jul 4. J Invertebr Pathol. 2008. PMID: 17689558

• Overview on current status of biotechnological interventions on yellow stem borer Scirpophaga incertulas (Lepidoptera: Crambidae) resistance in rice.

  Deka S, Barthakur S. Deka S, et al. Biotechnol Adv. 2010 Jan-Feb;28(1):70-81. doi: 10.1016/j.biotechadv.2009.09.003. Biotechnol Adv. 2010. PMID: 19811767 Review.

• Insect-resistant genetically modified rice in China: from research to commercialization.

  Chen M, Shelton A, Ye GY. Chen M, et al. Annu Rev Entomol. 2011;56:81-101. doi: 10.1146/annurev-ento-120709-144810. Annu Rev Entomol. 2011. PMID: 20868281 Review.

Cited by

• Cloning, characterization, and expression of a new cry1Ab gene from DOR Bt-1, an indigenous isolate of Bacillus thuringiensis.

  Reddy VP, Rao NN, Devi PS, Sivaramakrishnan S, Narasu ML, Kumar VD. Reddy VP, et al. Mol Biotechnol. 2013 Jul;54(3):795-802. doi: 10.1007/s12033-012-9627-3. Mol Biotechnol. 2013. PMID: 23224937

• Optimization of the cry1Ah1 Sequence Enhances the Hyper-Resistance of Transgenic Poplars to Hyphantria cunea.

  Xu C, Wei H, Wang L, Yin T, Zhuge Q. Xu C, et al. Front Plant Sci. 2019 Mar 26;10:335. doi: 10.3389/fpls.2019.00335. eCollection 2019. Front Plant Sci. 2019. PMID: 30972085 Free PMC article.

• A 90-day subchronic feeding study of genetically modified rice expressing Cry1Ab protein in Sprague-Dawley rats.

  Song H, He X, Zou S, Zhang T, Luo Y, Huang K, Zhu Z, Xu W. Song H, et al. Transgenic Res. 2015 Apr;24(2):295-308. doi: 10.1007/s11248-014-9844-6. Epub 2014 Nov 1. Transgenic Res. 2015. PMID: 25367203

• Binding site concentration explains the differential susceptibility of Chilo suppressalis and Sesamia inferens to Cry1A-producing rice.

  Han L, Han C, Liu Z, Chen F, Jurat-Fuentes JL, Hou M, Peng Y. Han L, et al. Appl Environ Microbiol. 2014 Aug;80(16):5134-40. doi: 10.1128/AEM.01544-14. Epub 2014 Jun 13. Appl Environ Microbiol. 2014. PMID: 24928872 Free PMC article.

• When the Whole is Not Greater than the Sum of the Parts: A Critical Review of Laboratory Bioassay Effects Testing for Insecticidal Protein Interactions.

  Walters FS, Graser G, Burns A, Raybould A. Walters FS, et al. Environ Entomol. 2018 Apr 5;47(2):484-497. doi: 10.1093/ee/nvx207. Environ Entomol. 2018. PMID: 29432611 Free PMC article. Review.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Other Literature Sources

  • The Lens - Patent Citations Database