In vivo transcriptional pattern in the infC operon of Bacillus stearothermophilus - PubMed
In vivo transcriptional pattern in the infC operon of Bacillus stearothermophilus
M Falconi et al. Mol Gen Genet. 1991 May.
Abstract
By Northern blot and primer extension analyses it was shown that in Bacillus stearothermophilus the genes infC, rpmI and rplT constitute a single transcriptional unit; the promoter and the transcriptional start-point used in vivo were identified and the half-life of the transcript (1.2 min) was determined. No indication of multiple initiation sites nor of differential stability of different regions of the transcript was found. The results suggest that Escherichia coli and B. stearothermophilus have a different pattern of transcription around the infC gene cluster and that differential translational efficiency within the infC-rpmI-rplT transcriptional unit accounts for the different levels of IF3, L35 and L20 expression. The rare AUU initiation codon is the only strictly conserved element of the several peculiar transcriptional and translational features found in E. coli infC. Upon changing this codon to AUG, we found a ca. 30-fold increased expression of B. stearothermophilus infC, which is similar to that previously found with E. coli infC (i.e. 40-fold), and provided evidence that regulation of infC expression through its rare AUU initiation codon might be a general phenomenon in bacteria.
Similar articles
-
Translational regulation of infC operon in Bacillus stearothermophilus.
Pediconi D, Spurio R, LaTeana A, Jemiolo D, Gualerzi CO, Pon CL. Pediconi D, et al. Biochem Cell Biol. 1995 Nov-Dec;73(11-12):1071-8. doi: 10.1139/o95-115. Biochem Cell Biol. 1995. PMID: 8722023 Review.
-
Translated translational operator in Escherichia coli. Auto-regulation in the infC-rpmI-rplT operon.
Lesage P, Truong HN, Graffe M, Dondon J, Springer M. Lesage P, et al. J Mol Biol. 1990 Jun 5;213(3):465-75. doi: 10.1016/S0022-2836(05)80208-6. J Mol Biol. 1990. PMID: 2191140
-
Hu WS, Wang RY, Shih JW, Lo SC. Hu WS, et al. Gene. 1993 May 15;127(1):79-85. doi: 10.1016/0378-1119(93)90619-e. Gene. 1993. PMID: 8486291
-
Pon CL, Brombach M, Thamm S, Gualerzi CO. Pon CL, et al. Mol Gen Genet. 1989 Aug;218(2):355-7. doi: 10.1007/BF00331290. Mol Gen Genet. 1989. PMID: 2779520
-
Lesage P, Chiaruttini C, Graffe M, Dondon J, Milet M, Springer M. Lesage P, et al. J Mol Biol. 1992 Nov 20;228(2):366-86. doi: 10.1016/0022-2836(92)90827-7. J Mol Biol. 1992. PMID: 1453449
Cited by
-
La Teana A, Pon CL, Gualerzi CO. La Teana A, et al. Proc Natl Acad Sci U S A. 1993 May 1;90(9):4161-5. doi: 10.1073/pnas.90.9.4161. Proc Natl Acad Sci U S A. 1993. PMID: 8483930 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources