Estrogen configures sexual dimorphism in the preoptic area of C57BL/6J and ddN strains of mice - PubMed

Affiliations

• PMID: 20593361
• DOI: 10.1002/cne.22419

Estrogen configures sexual dimorphism in the preoptic area of C57BL/6J and ddN strains of mice

Chitose Orikasa et al. J Comp Neurol. 2010.

Abstract

Immunohistochemistry using a calbindin D28k antibody revealed a marked sex difference in neuronal distribution in the central portion of the medial preoptic area in C57BL/6J and ddN strains of mice when the animals were sacrificed on D65 (D1 = the day of birth). Male mice had a distinct ellipsoidal cell aggregate, whereas females lacked such a structure. This sex difference was not observed in Nissl-stained sections. Co-localization of calbindin D28k and the neuron-specific nuclear protein NeuN confrmed that the cells in the aggregate were neurons. The aggregates were larger in males than in females in both strains. When observed on D65, males orchidectomized on D1 had smaller aggregates. However, daily injections of 2 microg estradiol benzoate through D1-D5 as well as a single injection of 100 microg testosterone propionate on D1 enlarged the aggregates in females, but a single injection of 100 microg dihydrotestosterone on D1 had no effect on the female phenotype. Similar endocrine manipulations had no effects in adult animals of both sexes. Thus, the calbindin-immunoreactive cell aggregates in the preoptic area of C57BL/6J and ddN mice are homologous to the sexually dimorphic nucleus of the rat preoptic area in terms of the morphology and sex steroid-dependent organization.

Similar articles

• Differential disruption of nuclear volume and neuronal phenotype in the preoptic area by neonatal exposure to genistein and bisphenol-A.

  Patisaul HB, Fortino AE, Polston EK. Patisaul HB, et al. Neurotoxicology. 2007 Jan;28(1):1-12. doi: 10.1016/j.neuro.2006.10.001. Epub 2006 Oct 17. Neurotoxicology. 2007. PMID: 17109964

• Transient transcription of the somatostatin gene at the time of estrogen-dependent organization of the sexually dimorphic nucleus of the rat preoptic area.

  Orikasa C, Kondo Y, Sakuma Y. Orikasa C, et al. Endocrinology. 2007 Mar;148(3):1144-9. doi: 10.1210/en.2006-1214. Epub 2006 Nov 30. Endocrinology. 2007. PMID: 17138650

• Evidence for the existence of an estrogen-responsive sexually dimorphic group of cells in the medial preoptic area of the 129SvEv mouse strain.

  Hill RA, Simpson ER, Boon WC. Hill RA, et al. Int J Impot Res. 2008 May-Jun;20(3):315-23. doi: 10.1038/ijir.2008.2. Epub 2008 Feb 14. Int J Impot Res. 2008. PMID: 18273027

• The sexually dimorphic medial preoptic nucleus of quail: a key brain area mediating steroid action on male sexual behavior.

  Panzica GC, Viglietti-Panzica C, Balthazart J. Panzica GC, et al. Front Neuroendocrinol. 1996 Jan;17(1):51-125. doi: 10.1006/frne.1996.0002. Front Neuroendocrinol. 1996. PMID: 8788569 Review.

• Development of the sexually dimorphic nucleus of the preoptic area and the influence of estrogen-like compounds.

  He Z, Ferguson SA, Cui L, Greenfield LJ, Paule MG. He Z, et al. Neural Regen Res. 2013 Oct 15;8(29):2763-74. doi: 10.3969/j.issn.1673-5374.2013.29.008. Neural Regen Res. 2013. PMID: 25206587 Free PMC article. Review.

Cited by

• In vivo and in vitro sex differences in the dendritic morphology of developing murine hippocampal and cortical neurons.

  Keil KP, Sethi S, Wilson MD, Chen H, Lein PJ. Keil KP, et al. Sci Rep. 2017 Aug 16;7(1):8486. doi: 10.1038/s41598-017-08459-z. Sci Rep. 2017. PMID: 28814778 Free PMC article.

• Circuit and synaptic organization of forebrain-to-midbrain pathways that promote and suppress vocalization.

  Michael V, Goffinet J, Pearson J, Wang F, Tschida K, Mooney R. Michael V, et al. Elife. 2020 Dec 29;9:e63493. doi: 10.7554/eLife.63493. Elife. 2020. PMID: 33372655 Free PMC article.

• Sexually Dimorphic Formation of the Preoptic Area and the Bed Nucleus of the Stria Terminalis by Neuroestrogens.

  Tsukahara S, Morishita M. Tsukahara S, et al. Front Neurosci. 2020 Jul 29;14:797. doi: 10.3389/fnins.2020.00797. eCollection 2020. Front Neurosci. 2020. PMID: 32848568 Free PMC article. Review.

• Cellular Composition of the Preoptic Area Regulating Sleep, Parental, and Sexual Behavior.

  Tsuneoka Y, Funato H. Tsuneoka Y, et al. Front Neurosci. 2021 Mar 31;15:649159. doi: 10.3389/fnins.2021.649159. eCollection 2021. Front Neurosci. 2021. PMID: 33867927 Free PMC article. Review.

• Two-Step Actions of Testicular Androgens in the Organization of a Male-Specific Neural Pathway from the Medial Preoptic Area to the Ventral Tegmental Area for Modulating Sexually Motivated Behavior.

  Morishita M, Kobayashi K, Mitsuzuka M, Takagi R, Ono K, Momma R, Tsuneoka Y, Horio S, Tsukahara S. Morishita M, et al. J Neurosci. 2023 Nov 1;43(44):7322-7336. doi: 10.1523/JNEUROSCI.0361-23.2023. Epub 2023 Sep 18. J Neurosci. 2023. PMID: 37722849 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Ovid Technologies, Inc.
  • Wiley

• Molecular Biology Databases

  • Mouse Genome Informatics (MGI)

• Research Materials

  • National BioResource Project

• Miscellaneous

  • SciCrunch