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Constructing ensembles for intrinsically disordered proteins - PubMed

Review

Constructing ensembles for intrinsically disordered proteins

Charles K Fisher et al. Curr Opin Struct Biol. 2011 Jun.

Abstract

The relatively flat energy landscapes associated with intrinsically disordered proteins makes modeling these systems especially problematic. A comprehensive model for these proteins requires one to build an ensemble consisting of a finite collection of structures, and their corresponding relative stabilities, which adequately capture the range of accessible states of the protein. In this regard, methods that use computational techniques to interpret experimental data in terms of such ensembles are an essential part of the modeling process. In this review, we critically assess the advantages and limitations of current techniques and discuss new methods for the validation of these ensembles.

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Figures

**Figure 1**
The energy landscape of a folded protein (A) exhibits a well-defined minimum energy state corresponding to the folded conformation. In comparison, the energy landscape of an IDP (B) lacks a deep energy minimum.

**Figure 2**
A schematic representation of the Reference Ensemble Method for validating ensemble construction algorithms.

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Constructing ensembles for intrinsically disordered proteins - PubMed