ChREBP expression in the liver, adipose tissue and differentiated preadipocytes in human obesity - PubMed

Affiliations

• PMID: 21840420
• DOI: 10.1016/j.bbalip.2011.07.016

Free article

ChREBP expression in the liver, adipose tissue and differentiated preadipocytes in human obesity

Carmen Hurtado del Pozo et al. Biochim Biophys Acta. 2011 Dec.

Free article

Abstract

ChREBP is an essential transcription factor for lipogenesis. Its physiological role in adipose tissue has been studied only to a small extent and the control of its expression remains unknown in human adipocytes. We have studied ChREBP mRNA and protein expression levels in the liver and the omental (OM) and subcutaneous (SC) adipose tissues from obese and lean subjects, as well as in human differentiated preadipocytes. Liver and OM and SC adipose tissue biopsies were obtained from lean and obese patients. Human preadipocytes were isolated from the adipose tissues from obese patients and differentiated under adipogenic conditions. ChREBP expression levels were quantified by RT-PCR and Western blot analysis. We found opposing results in terms of ChREBP regulation in the liver and adipose samples. ChREBP increased in the liver from obese compared to lean subjects, whereas the expression decreased in both adipose tissues. The mRNAs of other adipogenic markers were checked in these tissues. The pattern of FASN was similar to the one for ChREBP, ADCY3 decreased in both adipose tissues from obese patients, AP2 decreased only in OM adipose tissue of obese patients and ATGL did not change. The levels of ChREBP mRNA and protein showed dramatic increases during the differentiation of human OM and SC preadipocytes. In conclusion, ChREBP expression has an opposite regulation in the liver and adipose tissue from obese subjects which is compatible with the increased hepatic lipogenesis and decreased adipocytic lipogenesis found in these patients. The dramatic increase of ChREBP mRNA and protein levels during preadipocyte differentiation suggests a role in adipogenesis.

Similar articles

• Thyroid hormone responsive Spot 14 increases during differentiation of human adipocytes and its expression is down-regulated in obese subjects.

  Ortega FJ, Vazquez-Martin A, Moreno-Navarrete JM, Bassols J, Rodriguez-Hermosa J, Gironés J, Ricart W, Peral B, Tinahones FJ, Fruhbeck G, Menendez JA, Fernández-Real JM. Ortega FJ, et al. Int J Obes (Lond). 2010 Mar;34(3):487-99. doi: 10.1038/ijo.2009.263. Epub 2009 Dec 22. Int J Obes (Lond). 2010. PMID: 20029374

• Cyanidin reduces preadipocyte differentiation and relative ChREBP expression.

  Pompei A, Toniato E, Innocenti P, D Alimonte I, Cellini C, Mattoscio D, Cotellese R, Bosco D, Ciccarelli R, Dadorante V, D Orazio N, Martinotti S, Robuffo I. Pompei A, et al. J Biol Regul Homeost Agents. 2012 Apr-Jun;26(2):253-64. J Biol Regul Homeost Agents. 2012. PMID: 22824753

• Human growth hormone receptor (GHR) expression in obesity: I. GHR mRNA expression in omental and subcutaneous adipose tissues of obese women.

  Erman A, Veilleux A, Tchernof A, Goodyer CG. Erman A, et al. Int J Obes (Lond). 2011 Dec;35(12):1511-9. doi: 10.1038/ijo.2011.23. Epub 2011 Mar 8. Int J Obes (Lond). 2011. PMID: 21386804

• Recent progress on the role of ChREBP in glucose and lipid metabolism.

  Iizuka K. Iizuka K. Endocr J. 2013;60(5):543-55. doi: 10.1507/endocrj.ej13-0121. Epub 2013 Apr 19. Endocr J. 2013. PMID: 23604004 Review.

• Adipose tissue regulates insulin sensitivity: role of adipogenesis, de novo lipogenesis and novel lipids.

  Smith U, Kahn BB. Smith U, et al. J Intern Med. 2016 Nov;280(5):465-475. doi: 10.1111/joim.12540. Epub 2016 Oct 3. J Intern Med. 2016. PMID: 27699898 Free PMC article. Review.

Cited by

• Recent advances in the pathogenesis of hereditary fructose intolerance: implications for its treatment and the understanding of fructose-induced non-alcoholic fatty liver disease.

  Buziau AM, Schalkwijk CG, Stehouwer CDA, Tolan DR, Brouwers MCGJ. Buziau AM, et al. Cell Mol Life Sci. 2020 May;77(9):1709-1719. doi: 10.1007/s00018-019-03348-2. Epub 2019 Nov 12. Cell Mol Life Sci. 2020. PMID: 31713637 Free PMC article. Review.

• Map4k4 suppresses Srebp-1 and adipocyte lipogenesis independent of JNK signaling.

  Danai LV, Guilherme A, Guntur KV, Straubhaar J, Nicoloro SM, Czech MP. Danai LV, et al. J Lipid Res. 2013 Oct;54(10):2697-707. doi: 10.1194/jlr.M038802. Epub 2013 Aug 7. J Lipid Res. 2013. PMID: 23924694 Free PMC article.

• Experimental Nonalcoholic Steatohepatitis and Liver Fibrosis Are Ameliorated by Pharmacologic Activation of Nrf2 (NF-E2 p45-Related Factor 2).

  Sharma RS, Harrison DJ, Kisielewski D, Cassidy DM, McNeilly AD, Gallagher JR, Walsh SV, Honda T, McCrimmon RJ, Dinkova-Kostova AT, Ashford MLJ, Dillon JF, Hayes JD. Sharma RS, et al. Cell Mol Gastroenterol Hepatol. 2017 Dec 13;5(3):367-398. doi: 10.1016/j.jcmgh.2017.11.016. eCollection 2018 Mar. Cell Mol Gastroenterol Hepatol. 2017. PMID: 29552625 Free PMC article.

• Liver sympathetic denervation reverses obesity-induced hepatic steatosis.

  Hurr C, Simonyan H, Morgan DA, Rahmouni K, Young CN. Hurr C, et al. J Physiol. 2019 Sep;597(17):4565-4580. doi: 10.1113/JP277994. Epub 2019 Jul 26. J Physiol. 2019. PMID: 31278754 Free PMC article.

• De novo lipogenesis in human fat and liver is linked to ChREBP-β and metabolic health.

  Eissing L, Scherer T, Tödter K, Knippschild U, Greve JW, Buurman WA, Pinnschmidt HO, Rensen SS, Wolf AM, Bartelt A, Heeren J, Buettner C, Scheja L. Eissing L, et al. Nat Commun. 2013;4:1528. doi: 10.1038/ncomms2537. Nat Commun. 2013. PMID: 23443556 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Digital CSIC Spanish National Research Council
  • Elsevier Science

• Other Literature Sources

  • The Lens - Patent Citations Database

• Medical

  • MedlinePlus Health Information

• Research Materials

  • NCI CPTC Antibody Characterization Program

• Miscellaneous

  • NCI CPTAC Assay Portal