Protein expression and purification of integrin I domains and IgSF ligands for crystallography - PubMed

Protein expression and purification of integrin I domains and IgSF ligands for crystallography

Hongmin Zhang et al. Methods Mol Biol. 2012.

Abstract

Cell adhesion depends on combinational expression and interactions of a large number of adhesion molecules at cell-to-cell or cell-to-matrix contact sites. Integrins and their immunoglobulin superfamily (IgSF) ligands represent foremost classes of cell adhesion molecules in immune system. Structural study is critical for a better understanding of the interactions between integrins and their IgSF ligands. Here we describe protocols for protein expression of integrin αL I domain and its IgSF ligand ICAM-5 D1D2 fragment for crystallography.

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