Analysis of histones and histone variants in plants - PubMed
Analysis of histones and histone variants in plants
Ila Trivedi et al. Methods Mol Biol. 2012.
Abstract
Histone proteins are the major protein components of chromatin - the physiologically relevant form of the genome (or epigenome) in all eukaryotic cells. For many years, histones were considered passive structural components of eukaryotic chromatin. In recent years, it has been demonstrated that dynamic association of histones and their variants to the genome plays a very important role in gene regulation. Histones are extensively modified during posttranslation viz. acetylation, methylation, phosphorylation, ubiquitylation, etc., and the identification of these covalent marks on canonical and variant histones is crucial for the understanding of their biological significance. Different biochemical techniques have been developed to purify and separate histone proteins; here, we describe techniques for analysis of histones from plant tissues.
Similar articles
-
Extraction, purification and analysis of histones.
Shechter D, Dormann HL, Allis CD, Hake SB. Shechter D, et al. Nat Protoc. 2007;2(6):1445-57. doi: 10.1038/nprot.2007.202. Nat Protoc. 2007. PMID: 17545981
-
Analysis of histones, histone variants, and their post-translationally modified forms.
Lindner HH. Lindner HH. Electrophoresis. 2008 Jun;29(12):2516-32. doi: 10.1002/elps.200800094. Electrophoresis. 2008. PMID: 18494025 Review.
-
The malaria parasite Plasmodium falciparum histones: organization, expression, and acetylation.
Miao J, Fan Q, Cui L, Li J, Li J, Cui L. Miao J, et al. Gene. 2006 Mar 15;369:53-65. doi: 10.1016/j.gene.2005.10.022. Epub 2006 Jan 10. Gene. 2006. PMID: 16410041
-
Bergmüller E, Gehrig PM, Gruissem W. Bergmüller E, et al. J Proteome Res. 2007 Sep;6(9):3655-68. doi: 10.1021/pr0702159. Epub 2007 Aug 11. J Proteome Res. 2007. PMID: 17691833
-
Simple histone acetylation plays a complex role in the regulation of gene expression.
Fukuda H, Sano N, Muto S, Horikoshi M. Fukuda H, et al. Brief Funct Genomic Proteomic. 2006 Sep;5(3):190-208. doi: 10.1093/bfgp/ell032. Brief Funct Genomic Proteomic. 2006. PMID: 16980317 Review.
Cited by
-
Jiang Z, Jin F, Shan X, Li Y. Jiang Z, et al. Int J Mol Sci. 2019 Nov 26;20(23):5956. doi: 10.3390/ijms20235956. Int J Mol Sci. 2019. PMID: 31779286 Free PMC article.
-
Trivedi I, Ranjan A, Sharma YK, Sawant S. Trivedi I, et al. Protein J. 2012 Aug;31(6):477-86. doi: 10.1007/s10930-012-9425-6. Protein J. 2012. PMID: 22644313
-
Waititu JK, Zhang X, Chen T, Zhang C, Zhao Y, Wang H. Waititu JK, et al. Int J Mol Sci. 2021 Jun 29;22(13):6980. doi: 10.3390/ijms22136980. Int J Mol Sci. 2021. PMID: 34209553 Free PMC article.
-
Ashraf JM, Rabbani G, Ahmad S, Hasan Q, Khan RH, Alam K, Choi I. Ashraf JM, et al. PLoS One. 2015 Jun 29;10(6):e0130630. doi: 10.1371/journal.pone.0130630. eCollection 2015. PLoS One. 2015. PMID: 26121680 Free PMC article.
-
Drought-Responsive Mechanisms in Plant Leaves Revealed by Proteomics.
Wang X, Cai X, Xu C, Wang Q, Dai S. Wang X, et al. Int J Mol Sci. 2016 Oct 18;17(10):1706. doi: 10.3390/ijms17101706. Int J Mol Sci. 2016. PMID: 27763546 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources