Towards a structural basis for the relationship between blood group and the severity of El Tor cholera - PubMed
- ️Sun Jan 01 2012
. 2012 May 21;51(21):5143-6.
doi: 10.1002/anie.201109068. Epub 2012 Apr 5.
Affiliations
- PMID: 22488789
- PMCID: PMC3505909
- DOI: 10.1002/anie.201109068
Free PMC article
Towards a structural basis for the relationship between blood group and the severity of El Tor cholera
Pintu K Mandal et al. Angew Chem Int Ed Engl. 2012.
Free PMC article
No abstract available
Figures
a) Plan and b) elevation views of cholera toxin B-subunit (CTB) with GM1 oligosaccharide 2 bound (3CHB.pdb). c) Elevation view of E. coli heat-labile toxin B-subunit (LTB) with blood group A oligosaccharide 3 b analog bound (2O2L.pdb).
Synthesis of oligosaccharides 7 and 8.
Representative examples of ITC experiments: a) Lewis-y 7 and b) B-Lewis-y 8 titrated into a mixture of LTBh (100 μ
m) and GM1os (200 μ
m); c) Lewis-y 7 and d) B-Lewis-y 8 titrated into a solution of El Tor CTB (100 μ
m). Raw titration data (black) and ligand dilution experiments (red) are shown in the top panels. Bottom panels display the integrated heat data with lines of best fit for each binding curve. c, d, g–j) Saturation transfer difference NMR (STD-NMR) spectra for each titration mixture.
Overlay of LTBh structure (light blue; 2O2L.pdb) and a model of El Tor CTB (yellow) constructed using the structure of classical biotype CTB mutant (3CHB.pdb) by introducing appropriate mutations at positions 18, 47 and 94. The position of oligosaccharide 4 (green) is based on the coordinates of analog 3 b bound to LTBh (2O2L.pdb). Q3′ and N/S4′ are residues in the neighboring B-subunit.
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