Molecular characterization and expression analysis of sodium pump genes in the marine red alga Porphyra yezoensis - PubMed

Affiliations

• PMID: 22531934
• DOI: 10.1007/s11033-012-1643-7

Molecular characterization and expression analysis of sodium pump genes in the marine red alga Porphyra yezoensis

Toshiki Uji et al. Mol Biol Rep. 2012 Aug.

Abstract

Sodium pumps (EC 3.6.3.9, Na(+)-ATPase), which mediate excretion of Na(+) from the cell, play a crucial role in Na(+) homeostasis in eukaryotic cells. The objective of this study is to understand the Na(+) efflux system in a marine red alga. We identified a novel sodium pump gene, PyKPA2, from the marine red alga Porphyra yezoensis. The amino acid sequence of PyKPA2 shares 65 % identity with PyKPA1, a previously identified P. yezoensis sodium pump. Similar to PyKPA1, PyKPA2 contains conserved sequences for functions such as phosphorylation, ATP binding, and cation binding. Phylogenetic analysis revealed that the two genes cluster with sodium pumps from algae. Reverse-transcription polymerase chain reaction (RT-PCR) analysis showed that PyKPA1 is expressed preferentially in sporophytes, whereas PyKPA2 is expressed specifically in gametophytes. RT-PCR and quantitative real-time PCR analysis revealed that PyKPA1 and PyKPA2 transcripts were upregulated and downregulated, respectively, in gametophytes during exposure to alkali stress. In addition, transcription of both genes in gametophytes was also induced by cold stress. These results suggest that PyKPA1 and PyKPA2 play an important role in alkali and cold stress tolerance.

Similar articles

• Characterization of the sporophyte-preferential gene promoter from the red alga Porphyra yezoensis using transient gene expression.

  Uji T, Mizuta H, Saga N. Uji T, et al. Mar Biotechnol (NY). 2013 Apr;15(2):188-96. doi: 10.1007/s10126-012-9475-y. Epub 2012 Aug 4. Mar Biotechnol (NY). 2013. PMID: 22865243

• Sodium, potassium-atpases in algae and oomycetes.

  Barrero-Gil J, Garciadeblás B, Benito B. Barrero-Gil J, et al. J Bioenerg Biomembr. 2005 Aug;37(4):269-78. doi: 10.1007/s10863-005-6637-x. J Bioenerg Biomembr. 2005. PMID: 16167182

• Reverse transcriptase-like sequences related to retrotransposon in a red alga, Porphyra yezoensis.

  Zhang W, Sakai M, Lin X, Takechi K, Takano H, Takio S. Zhang W, et al. Biosci Biotechnol Biochem. 2006 Aug;70(8):1999-2003. doi: 10.1271/bbb.60118. Biosci Biotechnol Biochem. 2006. PMID: 16926517

• Sodium or potassium efflux ATPase a fungal, bryophyte, and protozoal ATPase.

  Rodríguez-Navarro A, Benito B. Rodríguez-Navarro A, et al. Biochim Biophys Acta. 2010 Oct;1798(10):1841-53. doi: 10.1016/j.bbamem.2010.07.009. Epub 2010 Jul 19. Biochim Biophys Acta. 2010. PMID: 20650263 Review.

• Porphyra: a marine crop shaped by stress.

  Blouin NA, Brodie JA, Grossman AC, Xu P, Brawley SH. Blouin NA, et al. Trends Plant Sci. 2011 Jan;16(1):29-37. doi: 10.1016/j.tplants.2010.10.004. Epub 2010 Nov 8. Trends Plant Sci. 2011. PMID: 21067966 Review.

Cited by

• Development of Chromatin Immunoprecipitation for the Analysis of Histone Modifications in Red Macroalga Neopyropia yezoensis (Rhodophyta).

  Ueda S, Mizuta H, Uji T. Ueda S, et al. Mol Biotechnol. 2023 Apr;65(4):590-597. doi: 10.1007/s12033-022-00562-5. Epub 2022 Sep 13. Mol Biotechnol. 2023. PMID: 36098867

• Interaction Network Construction and Functional Analysis of the Plasma Membrane H⁺-ATPase in Bangia fuscopurpurea (Rhodophyta).

  Yao H, Wang W, Cao Y, Liang Z, Zhang P. Yao H, et al. Int J Mol Sci. 2023 Apr 21;24(8):7644. doi: 10.3390/ijms24087644. Int J Mol Sci. 2023. PMID: 37108805 Free PMC article.

• Chemoprotective effects of a recombinant protein from Pyropia yezoensis and synthetic peptide against acetaminophen-induced Chang liver cell death.

  Choi YH, Kim EY, Mikami K, Nam TJ. Choi YH, et al. Int J Mol Med. 2015 Aug;36(2):369-76. doi: 10.3892/ijmm.2015.2253. Epub 2015 Jun 19. Int J Mol Med. 2015. PMID: 26096530 Free PMC article.

• Characterization and expression profiles of small heat shock proteins in the marine red alga Pyropia yezoensis.

  Uji T, Gondaira Y, Fukuda S, Mizuta H, Saga N. Uji T, et al. Cell Stress Chaperones. 2019 Jan;24(1):223-233. doi: 10.1007/s12192-018-00959-9. Epub 2019 Jan 10. Cell Stress Chaperones. 2019. PMID: 30632066 Free PMC article.

• Characterization of the sporophyte-preferential gene promoter from the red alga Porphyra yezoensis using transient gene expression.

  Uji T, Mizuta H, Saga N. Uji T, et al. Mar Biotechnol (NY). 2013 Apr;15(2):188-96. doi: 10.1007/s10126-012-9475-y. Epub 2012 Aug 4. Mar Biotechnol (NY). 2013. PMID: 22865243

References

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Springer

• Research Materials

  • NCI CPTC Antibody Characterization Program