Genome-wide mapping of nucleosome occupancy, histone modifications, and gene expression using next-generation sequencing technology - PubMed

Genome-wide mapping of nucleosome occupancy, histone modifications, and gene expression using next-generation sequencing technology

Gang Wei et al. Methods Enzymol. 2012.

Abstract

Gene transcription can be regulated through alteration of chromatin structure, such as changes in nucleosome positioning and histone-modification patterns. Recent development of techniques based on the next-generation sequencing technology has allowed high-resolution analysis of genome-wide distribution of these chromatin features. In this chapter, we describe in detail the protocols of ChIP-Seq and MNase-Seq, which have been developed to detect the genome-wide profiles of transcription factor binding, histone modifications, and nucleosome occupancy. We also describe RNA-Seq protocols used to map global gene expression profiles.

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