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The tooth for molecular analysis and identification : a forensic approach - PubMed

  • ️Sun Jan 01 2012

The tooth for molecular analysis and identification : a forensic approach

A Corte-Real et al. J Forensic Odontostomatol. 2012.

Abstract

The aim of this study is to optimize laboratory preparation of teeth for DNA identification. By sectioning the tooth topographically into two different radicular portions, it was analyzed whether these portions of mineralized tissue differ in the quantity and quality of DNA they contain. 25 teeth were subject to different experimental conditions and total DNA was quantified for each individual tooth's radicular portion: apical and remaining root, according to a 2003 study by Gaytemenn and Sweet. We verified, with statistically significant figures, that the apical portion of the tooth is that which contains the greatest quantity of DNA. Different analytical procedures were studied for various polymorphic markers to evaluate the quality of the DNA. We concluded that the tooth is topographically distinct in both DNA quantity and quality. The tooth's apical portion is the preferential choice in sample preparation of dental mineralized tissue for molecular analysis and identification.

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Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1

Sequence of the used tooth, cuts by Gaytmenn and Sweet

Fig. 2
Fig. 2

The Gaytmenn and Sweet guidelines

Fig. 3
Fig. 3

Quantification results of the apical radicular portion and the remaining radicular portion, statistically analyzed with SPSS.

Fig. 4
Fig. 4

Microscopic photograph of cementogenesis in the apical root portion.

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