Sulfur-rich proteins of Chlamydia trachomatis: developmentally regulated transcription of polycistronic mRNA from tandem promoters - PubMed

Sulfur-rich proteins of Chlamydia trachomatis: developmentally regulated transcription of polycistronic mRNA from tandem promoters

P R Lambden et al. Gene. 1990.

Abstract

RNA was extracted at various times from cells infected with Chlamydia trachomatis serovar L1. Northern-blot analysis showed that transcription of the CrP gene encoding the 60-kDa cysteine-rich outer membrane protein (CrP) produces a temporally controlled polycistronic mRNA. Primer extension analysis indicated the presence of tandem promoters separated by 66 nt with transcriptional start points (tsp) located 577 and 643 nt upstream from the start codon of the mature 60-kDa CrP. Nucleotide (nt) sequencing of this region revealed a small open reading frame (SORF) with coding potential for an 88-amino acid protein containing 13 cysteine residues. This SORF is transcribed as both a polycistronic 2300-nt mRNA together with the CrP gene, and as a separate 480-nt mRNA. Analysis of the upstream sequences, around the tsp for these mRNAs, revealed the presence of three inverted repeat structures that might act as binding domain(s) for a regulatory protein.

Similar articles

• Heat shock response of murine Chlamydia trachomatis.

  Engel JN, Pollack J, Perara E, Ganem D. Engel JN, et al. J Bacteriol. 1990 Dec;172(12):6959-72. doi: 10.1128/jb.172.12.6959-6972.1990. J Bacteriol. 1990. PMID: 2254267 Free PMC article.

• Transcriptional analysis of the Chlamydia trachomatis plasmid pCT identifies temporally regulated transcripts, anti-sense RNA and sigma 70-selected promoters.

  Ricci S, Cevenini R, Cosco E, Comanducci M, Ratti G, Scarlato V. Ricci S, et al. Mol Gen Genet. 1993 Mar;237(3):318-26. doi: 10.1007/BF00279434. Mol Gen Genet. 1993. PMID: 7683369

• Developmental regulation of tandem promoters for the major outer membrane protein gene of Chlamydia trachomatis.

  Stephens RS, Wagar EA, Edman U. Stephens RS, et al. J Bacteriol. 1988 Feb;170(2):744-50. doi: 10.1128/jb.170.2.744-750.1988. J Bacteriol. 1988. PMID: 2448291 Free PMC article.

• Molecular cloning and sequence analysis of a developmentally regulated cysteine-rich outer membrane protein from Chlamydia trachomatis.

  Clarke IN, Ward ME, Lambden PR. Clarke IN, et al. Gene. 1988 Nov 30;71(2):307-14. doi: 10.1016/0378-1119(88)90047-9. Gene. 1988. PMID: 3066701

• Identification by sequence analysis of two-site posttranslational processing of the cysteine-rich outer membrane protein 2 of Chlamydia trachomatis serovar L2.

  Allen JE, Stephens RS. Allen JE, et al. J Bacteriol. 1989 Jan;171(1):285-91. doi: 10.1128/jb.171.1.285-291.1989. J Bacteriol. 1989. PMID: 2914847 Free PMC article.

Cited by

• Topological analysis of Chlamydia trachomatis L2 outer membrane protein 2.

  Mygind P, Christiansen G, Birkelund S. Mygind P, et al. J Bacteriol. 1998 Nov;180(21):5784-7. doi: 10.1128/JB.180.21.5784-5787.1998. J Bacteriol. 1998. PMID: 9791135 Free PMC article.

• Interaction of outer envelope proteins of Chlamydia psittaci GPIC with the HeLa cell surface.

  Ting LM, Hsia RC, Haidaris CG, Bavoil PM. Ting LM, et al. Infect Immun. 1995 Sep;63(9):3600-8. doi: 10.1128/iai.63.9.3600-3608.1995. Infect Immun. 1995. PMID: 7642297 Free PMC article.

• Enhancement of in vitro transcription by addition of cloned, overexpressed major sigma factor of Chlamydia psittaci 6BC.

  Douglas AL, Saxena NK, Hatch TP. Douglas AL, et al. J Bacteriol. 1994 May;176(10):3033-9. doi: 10.1128/jb.176.10.3033-3039.1994. J Bacteriol. 1994. PMID: 8188604 Free PMC article.

• Cloning and characterization of the RNA polymerase alpha-subunit operon of Chlamydia trachomatis.

  Tan M, Klein R, Grant R, Ganem D, Engel J. Tan M, et al. J Bacteriol. 1993 Nov;175(22):7150-9. doi: 10.1128/jb.175.22.7150-7159.1993. J Bacteriol. 1993. PMID: 8226662 Free PMC article.

• Lipooligosaccharide is required for the generation of infectious elementary bodies in Chlamydia trachomatis.

  Nguyen BD, Cunningham D, Liang X, Chen X, Toone EJ, Raetz CR, Zhou P, Valdivia RH. Nguyen BD, et al. Proc Natl Acad Sci U S A. 2011 Jun 21;108(25):10284-9. doi: 10.1073/pnas.1107478108. Epub 2011 May 31. Proc Natl Acad Sci U S A. 2011. PMID: 21628561 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Other Literature Sources

  • The Lens - Patent Citations Database

• Research Materials

  • NCI CPTC Antibody Characterization Program

• Miscellaneous

  • NCI CPTAC Assay Portal