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Evaluation of the relationship between GPR43 and adiposity in human - PubMed

  • ️Tue Jan 01 2013

Evaluation of the relationship between GPR43 and adiposity in human

Evelyne M Dewulf et al. Nutr Metab (Lond). 2013.

Abstract

Background: GPR43 is a G-protein-coupled receptor that participates in adipocyte differentiation in mice and is over-expressed in adipose tissue of obese mice. The aim of this study was to investigate the implication of GPR43 in adipogenesis in humans and to determine the influence of obesity on its expression in human adipose tissue.

Findings: Preadipocytes were isolated from human omental adipose tissue and cultured during 13 days. One PPARγ agonist (troglitazone) and three GPR43 agonists (two physiological and one synthetic) were tested for their ability to induce differentiation. After 13 days, the three GPR43 agonists had no impact on aP2 expression, a marker of adipocyte differentiation, whereas troglitazone led to a huge over-expression of aP2 in these cells but tended to decrease GPR43 expression (p=0.06).GPR43 and inflammatory markers expression was also quantified in omental adipose tissue from lean and obese individuals. GPR43 expression in total adipose tissue was similar between obese patients and lean subjects and did not correlate with aP2 expression. In contrast, GPR43 expression positively correlated with TNFα mRNA.

Conclusions: Our results suggest the absence of relationship between GPR43 and adipocyte differentiation in humans, unlike what was observed in mice. Furthermore, GPR43 expression is not increased in adipose tissue from obese subjects but could be related to TNFα-related inflammatory processes.

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Figures

Figure 1
Figure 1

Study of GPR43 involvement in human adipogenesis. GPR43 and aP2 mRNA levels in human preadipocytes, 13 days after induction of differentiation in presence of the vehicle (CT) or different pharmacological agents: troglitazone (TZD), acetate, propionate and CMTB. Mean value obtained in the CT group (i.e.; medium without the addition of the pharmacological agents) is set at 1. Data are mean ± SEM for 4 different cultures. Data with different superscript letters are significantly different with p<0.001, according to one-way ANOVA followed by post hoc Tukey’s multiple comparison test. GPR43: G-protein-coupled receptor 43.

Figure 2
Figure 2

Influence of obesity on GPR43 expression in human adipose tissue. A. GPR43, aP2, TNFα, CD68 and MCP-1 mRNA levels in human omental adipose tissue collected from 10 lean patients and 23 obese patients. Data are mean ± SEM, * p<0.01 according to the Student’s t-test. B. Correlations between GPR43 mRNA and TNFα, CD68 and MCP-1 mRNAs, r and p values according to the Pearson’s correlation test. GPR43: G-protein-coupled receptor 43; TNFα: tumor necrosis factor α; CD68: cluster of differentiation 68; MCP-1: monocyte chemoattractant protein-1.

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