Functional melanocytes are readily reprogrammable from multilineage-differentiating stress-enduring (muse) cells, distinct stem cells in human fibroblasts - PubMed

Affiliations

• PMID: 23563197
• DOI: 10.1038/jid.2013.172

Free article

Functional melanocytes are readily reprogrammable from multilineage-differentiating stress-enduring (muse) cells, distinct stem cells in human fibroblasts

Kenichiro Tsuchiyama et al. J Invest Dermatol. 2013 Oct.

Free article

Abstract

The induction of melanocytes from easily accessible stem cells has attracted attention for the treatment of melanocyte dysfunctions. We found that multilineage-differentiating stress-enduring (Muse) cells, a distinct stem cell type among human dermal fibroblasts, can be readily reprogrammed into functional melanocytes, whereas the remainder of the fibroblasts do not contribute to melanocyte differentiation. Muse cells can be isolated as cells positive for stage-specific embryonic antigen-3, a marker for undifferentiated human embryonic stem cells, and differentiate into cells representative of all three germ layers from a single cell, while also being nontumorigenic. The use of certain combinations of factors induces Muse cells to express melanocyte markers such as tyrosinase and microphthalmia-associated transcription factor and to show positivity for the 3,4-dihydroxy-L-phenylalanine reaction. When Muse cell-derived melanocytes were incorporated into three-dimensional (3D) cultured skin models, they localized themselves in the basal layer of the epidermis and produced melanin in the same manner as authentic melanocytes. They also maintained their melanin production even after the 3D cultured skin was transplanted to immunodeficient mice. This technique may be applicable to the efficient production of melanocytes from accessible human fibroblasts by using Muse cells, thereby contributing to autologous transplantation for melanocyte dysfunctions, such as vitiligo.

Similar articles

• Muse Cells Derived from Dermal Tissues Can Differentiate into Melanocytes.

  Tian T, Zhang RZ, Yang YH, Liu Q, Li D, Pan XR. Tian T, et al. Cell Reprogram. 2017 Apr;19(2):116-122. doi: 10.1089/cell.2016.0032. Epub 2017 Feb 7. Cell Reprogram. 2017. PMID: 28170296

• Artificial Pigmented Human Skin Created by Muse Cells.

  Yamauchi T, Yamasaki K, Tsuchiyama K, Aiba S. Yamauchi T, et al. Adv Exp Med Biol. 2018;1103:255-271. doi: 10.1007/978-4-431-56847-6_14. Adv Exp Med Biol. 2018. PMID: 30484234

• A quantitative analysis of multilineage-differentiating stress-enduring (Muse) cells in human adipose tissue and efficacy of melanocytes induction.

  Yamauchi T, Yamasaki K, Tsuchiyama K, Koike S, Aiba S. Yamauchi T, et al. J Dermatol Sci. 2017 Jun;86(3):198-205. doi: 10.1016/j.jdermsci.2017.03.001. Epub 2017 Mar 6. J Dermatol Sci. 2017. PMID: 28292562

• Pluripotent nontumorigenic multilineage differentiating stress enduring cells (Muse cells): a seven-year retrospective.

  Fisch SC, Gimeno ML, Phan JD, Simerman AA, Dumesic DA, Perone MJ, Chazenbalk GD. Fisch SC, et al. Stem Cell Res Ther. 2017 Oct 18;8(1):227. doi: 10.1186/s13287-017-0674-3. Stem Cell Res Ther. 2017. PMID: 29041955 Free PMC article. Review.

• The Muse Cell Discovery, Thanks to Wine and Science.

  Dezawa M. Dezawa M. Adv Exp Med Biol. 2018;1103:1-11. doi: 10.1007/978-4-431-56847-6_1. Adv Exp Med Biol. 2018. PMID: 30484221 Review.

Cited by

• Therapeutic Potential of Adipose-Derived SSEA-3-Positive Muse Cells for Treating Diabetic Skin Ulcers.

  Kinoshita K, Kuno S, Ishimine H, Aoi N, Mineda K, Kato H, Doi K, Kanayama K, Feng J, Mashiko T, Kurisaki A, Yoshimura K. Kinoshita K, et al. Stem Cells Transl Med. 2015 Feb;4(2):146-55. doi: 10.5966/sctm.2014-0181. Epub 2015 Jan 5. Stem Cells Transl Med. 2015. PMID: 25561682 Free PMC article.

• Elucidation of the enigma of glycosphingolipids in the regulation of inflammation and degeneration - Great progress over the last 70 years.

  Furukawa K, Ohmi Y, Kondo Y, Bhuiyan RH, Tajima O, Zhang P, Ohkawa Y, Furukawa K. Furukawa K, et al. Proc Jpn Acad Ser B Phys Biol Sci. 2019;95(3):136-149. doi: 10.2183/pjab.95.011. Proc Jpn Acad Ser B Phys Biol Sci. 2019. PMID: 30853699 Free PMC article. Review.

• Current and Future Perspectives of Stem Cell Therapy in Dermatology.

  Prodinger CM, Reichelt J, Bauer JW, Laimer M. Prodinger CM, et al. Ann Dermatol. 2017 Dec;29(6):667-687. doi: 10.5021/ad.2017.29.6.667. Epub 2017 Oct 30. Ann Dermatol. 2017. PMID: 29200755 Free PMC article. Review.

• Multilineage-differentiating stress-enduring cells: a powerful tool for tissue damage repair.

  Que H, Mai E, Hu Y, Li H, Zheng W, Jiang Y, Han F, Li X, Gong P, Gu J. Que H, et al. Front Cell Dev Biol. 2024 May 30;12:1380785. doi: 10.3389/fcell.2024.1380785. eCollection 2024. Front Cell Dev Biol. 2024. PMID: 38872932 Free PMC article. Review.

• Non-Tumorigenic Pluripotent Reparative Muse Cells Provide a New Therapeutic Approach for Neurologic Diseases.

  Yamashita T, Kushida Y, Abe K, Dezawa M. Yamashita T, et al. Cells. 2021 Apr 20;10(4):961. doi: 10.3390/cells10040961. Cells. 2021. PMID: 33924240 Free PMC article. Review.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Other Literature Sources

  • The Lens - Patent Citations Database
  • scite Smart Citations

• Medical

  • MedlinePlus Health Information