A bilirubin-inducible fluorescent protein from eel muscle - PubMed

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• PMID: 23768684
• DOI: 10.1016/j.cell.2013.05.038

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A bilirubin-inducible fluorescent protein from eel muscle

Akiko Kumagai et al. Cell. 2013.

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Abstract

The fluorescent protein toolbox has revolutionized experimental biology. Despite this advance, no fluorescent proteins have been identified from vertebrates, nor has chromogenic ligand-inducible activation or clinical utility been demonstrated. Here, we report the cloning and characterization of UnaG, a fluorescent protein from Japanese eel. UnaG belongs to the fatty-acid-binding protein (FABP) family, and expression in eel is restricted to small-diameter muscle fibers. On heterologous expression in cell lines or mouse brain, UnaG produces oxygen-independent green fluorescence. Remarkably, UnaG fluorescence is triggered by an endogenous ligand, bilirubin, a membrane-permeable heme metabolite and clinical health biomarker. The holoUnaG structure at 1.2 Å revealed a biplanar coordination of bilirubin by reversible π-conjugation, and we used this high-affinity and high-specificity interaction to establish a fluorescence-based human bilirubin assay with promising clinical utility. UnaG will be the prototype for a versatile class of ligand-activated fluorescent proteins, with applications in research, medicine, and bioengineering.

Comment in

• Fishing for fluorescent proteins.

  Eisenstein M. Eisenstein M. Nat Methods. 2013 Aug;10(8):695. doi: 10.1038/nmeth.2586. Nat Methods. 2013. PMID: 24058976 No abstract available.

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