Identification of sequences important in the nucleolar localization of human immunodeficiency virus Rev: relevance of nucleolar localization to function - PubMed

Identification of sequences important in the nucleolar localization of human immunodeficiency virus Rev: relevance of nucleolar localization to function

A W Cochrane et al. J Virol. 1990 Feb.

Abstract

The human immunodeficiency virus rev gene product regulates the expression of viral structural genes. It was recently shown that Rev regulates the export of viral structural mRNAs from the nucleus to the cytoplasm. Analysis of Rev subcellular localization reveals marked accumulation in the nucleolus, suggesting a role for the nucleolus in this export process. We report here the identification of amino acid residues critical to the nucleolar localization of Rev. Consistent with this finding, a Rev/beta-galactosidase fusion protein, harboring this region of Rev, localized entirely within the nucleolus. Of most significance, mutations that eliminated nucleolar localization markedly diminished Rev function, even though accumulation in the nucleoplasm was retained. These findings support a model whereby Rev-induced export of human immunodeficiency virus structural mRNAs from the nucleus to the cytoplasm is likely to involve nucleolar events.

Similar articles

• Inhibition of human immunodeficiency virus type 1 Rev function by a Rev mutant which interferes with nuclear/nucleolar localization of Rev.

  Kubota S, Furuta R, Maki M, Hatanaka M. Kubota S, et al. J Virol. 1992 Apr;66(4):2510-3. doi: 10.1128/JVI.66.4.2510-2513.1992. J Virol. 1992. PMID: 1548775 Free PMC article.

• Analysis of rev gene function on human immunodeficiency virus type 1 replication in lymphoid cells by using a quantitative polymerase chain reaction method.

  Arrigo SJ, Weitsman S, Rosenblatt JD, Chen IS. Arrigo SJ, et al. J Virol. 1989 Nov;63(11):4875-81. doi: 10.1128/JVI.63.11.4875-4881.1989. J Virol. 1989. PMID: 2677405 Free PMC article.

• Structural and functional analysis of the human immunodeficiency virus type 2 Rev protein.

  Dillon PJ, Nelbock P, Perkins A, Rosen CA. Dillon PJ, et al. J Virol. 1991 Jan;65(1):445-9. doi: 10.1128/JVI.65.1.445-449.1991. J Virol. 1991. PMID: 1985207 Free PMC article.

• Regulation of expression of human immunodeficiency virus.

  Pavlakis GN, Felber BK. Pavlakis GN, et al. New Biol. 1990 Jan;2(1):20-31. New Biol. 1990. PMID: 2078551 Review.

• The HIV-1 Rev protein.

  Pollard VW, Malim MH. Pollard VW, et al. Annu Rev Microbiol. 1998;52:491-532. doi: 10.1146/annurev.micro.52.1.491. Annu Rev Microbiol. 1998. PMID: 9891806 Review.

Cited by

• The bovine immunodeficiency virus Rev protein: identification of a novel nuclear import pathway and nuclear export signal among retroviral Rev/Rev-like proteins.

  Gomez Corredor A, Archambault D. Gomez Corredor A, et al. J Virol. 2012 May;86(9):4892-905. doi: 10.1128/JVI.05132-11. Epub 2012 Feb 29. J Virol. 2012. PMID: 22379104 Free PMC article.

• Direct participation of Sam68, the 68-kilodalton Src-associated protein in mitosis, in the CRM1-mediated Rev nuclear export pathway.

  Li J, Liu Y, Kim BO, He JJ. Li J, et al. J Virol. 2002 Aug;76(16):8374-82. doi: 10.1128/jvi.76.16.8374-8382.2002. J Virol. 2002. PMID: 12134041 Free PMC article.

• Structural Fluidity of the Human Immunodeficiency Virus Rev Response Element.

  Sherpa C, Grice SFJL. Sherpa C, et al. Viruses. 2020 Jan 11;12(1):86. doi: 10.3390/v12010086. Viruses. 2020. PMID: 31940828 Free PMC article. Review.

• Involvement of human CRM1 (exportin 1) in the export and multimerization of the Rex protein of human T-cell leukemia virus type 1.

  Hakata Y, Umemoto T, Matsushita S, Shida H. Hakata Y, et al. J Virol. 1998 Aug;72(8):6602-7. doi: 10.1128/JVI.72.8.6602-6607.1998. J Virol. 1998. PMID: 9658105 Free PMC article.

References

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central

• Other Literature Sources

  • The Lens - Patent Citations Database