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Urinary miR-29 correlates with albuminuria and carotid intima-media thickness in type 2 diabetes patients - PubMed

  • ️Tue Jan 01 2013

Urinary miR-29 correlates with albuminuria and carotid intima-media thickness in type 2 diabetes patients

Hui Peng et al. PLoS One. 2013.

Abstract

Background: Cell-free microRNAs stably and abundantly exist in body fluids and emerging evidence suggests cell-free microRNAs as novel and non-invasive disease biomarker. Deregulation of miR-29 is involved in the pathogenesis of diabetic nephropathy and insulin resistance thus may be implicated in diabetic vascular complication. Therefore, we investigated the possibility of urinary miR-29 as biomarker for diabetic nephropathy and atherosclerosis in patients with type 2 diabetes.

Methods: 83 patients with type 2 diabetes were enrolled in this study, miR-29a, miR-29b and miR-29c levels in urine supernatant was determined by TaqMan qRT-PCR, and a synthetic cel-miR-39 was added to the urine as a spike-in control before miRNAs extraction. Urinary albumin excretion rate and urine albumin/creatinine ratio, funduscopy and carotid ultrasound were used for evaluation of diabetic vascular complication. The laboratory parameters indicating blood glucose level, renal function and serum lipids were also collected.

Results: Patients with albuminuria (n = 42, age 60.62 ± 12.00 yrs) showed significantly higher comorbidity of diabetic retinopathy (p = 0.015) and higher levels of urinary miR-29a (p = 0.035) compared with those with normoalbuminuria (n = 41, age 58.54 ± 14.40 yrs). There was no significant difference in urinary miR-29b (p = 0.148) or miR-29c level (p = 0.321) between groups. Urinary albumin excretion rate significantly correlated with urinary miR-29a level (r = 0.286, p = 0.016), while urinary miR-29b significantly correlated with carotid intima-media thickness (cIMT) (r = 0.286, p = 0.046).

Conclusion: Urinary miR-29a correlated with albuminuria while urinary miR-29b correlated with carotid intima-media thickness (cIMT) in patients with type 2 diabetes. Therefore, they may have the potential to serve as alternative biomarker for diabetic nephropathy and atherosclerosis in type 2 diabetes.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Figure 1
Figure 1. The relative abundance of urinary miR-29 members in patients with Type 2 diabetes mellitus (n = 83).

Urinary miR-29a and miR-29c are significantly higher than miR-29b in type 2 diabetes patients (both with a p value <0.001).

Figure 2
Figure 2. Comparison of urinary miR-29 members between diabetes patients with albuminuria and normoalbuminuria.

Urinary miR-29a was higher in diabetes with albuminuria group than in diabetes with normoalbuminuria group (p = 0.035). There was no significant difference in urinary miR-29b (p = 0.148) or miR-29c (p = 0.321) levels between two groups. The values are represented as ratio to the median of diabetes with normoalbuminuria group. Data are compared by Mann-Whitney U test.

Figure 3
Figure 3. Correlation between urinary miR-29 members and urinary albumin excretion rate.

a: Urinary miR-29a significantly correlated with urinary albumin excretion rate (r = 0.286, p = 0.016). b: Correlation between urinary albumin excretion rate and miR-29b was borderline significant (r = 0.212, p = 0.078). c: There was no significant correlation between miR-29c and urinary albumin excretion rate (r = 0.151, p = 0.211). Data were compared by Spearman’s rank order correlations.

Figure 4
Figure 4. Correlation between urinary miR-29 members and carotid intima-media thickness (cIMT).

a: There was no significant correlation between urinary miR-29a and carotid intima-media thickness (cIMT) (r = 0.173, p = 0.234). b: Urinary miR-29b significantly correlated with cIMT (r = 0.286, p = 0.046). c: There was no significant correlation between urinary miR-29c and cIMT (r = 0.048, p = 0.741) levels. Data were compared by Spearman’s rank order correlations.

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