VSG gene 118 is transcribed from a cotransposed pol I-like promoter - PubMed
- ️Thu Jan 01 1987
VSG gene 118 is transcribed from a cotransposed pol I-like promoter
C Shea et al. Cell. 1987.
Free article
Abstract
To understand the control of differential variant cell surface glycoprotein (VSG) gene expression in T. brucei, we studied VSG gene and expression site transcription regulation. We show that the interchromosomal duplicative transposition of VSG gene 118, on an unusually large transposed segment, results in the transcriptional activation of a cotransposed RNA polymerase I-like (pol I) promoter, from which the VSG gene is transcribed. Transcription of VSG genes by pol I can therefore be regulated by DNA rearrangements that affect positional control of gene expression. A 5' cap is added in trans to the pol I-derived pre-mRNA, by addition of a pol II-derived 35 nucleotide mini-exon. A second gene (ESAG1) is located 25 kb upstream of the VSG 118 gene and is also transcribed. This expression site therefore contains at least two independently regulated genes. We discuss the putative importance of a nucleolar location for VSG gene and expression site transcription regulation.
Similar articles
-
Günzl A, Bruderer T, Laufer G, Schimanski B, Tu LC, Chung HM, Lee PT, Lee MG. Günzl A, et al. Eukaryot Cell. 2003 Jun;2(3):542-51. doi: 10.1128/EC.2.3.542-551.2003. Eukaryot Cell. 2003. PMID: 12796299 Free PMC article.
-
Nuclear repositioning of the VSG promoter during developmental silencing in Trypanosoma brucei.
Landeira D, Navarro M. Landeira D, et al. J Cell Biol. 2007 Jan 15;176(2):133-9. doi: 10.1083/jcb.200607174. Epub 2007 Jan 8. J Cell Biol. 2007. PMID: 17210949 Free PMC article.
-
De Lange T, Liu AY, Van der Ploeg LH, Borst P, Tromp MC, Van Boom JH. De Lange T, et al. Cell. 1983 Oct;34(3):891-900. doi: 10.1016/0092-8674(83)90546-9. Cell. 1983. PMID: 6313212
-
Transcription of protein-coding genes in trypanosomes by RNA polymerase I.
Lee MG, Van der Ploeg LH. Lee MG, et al. Annu Rev Microbiol. 1997;51:463-89. doi: 10.1146/annurev.micro.51.1.463. Annu Rev Microbiol. 1997. PMID: 9343357 Review.
-
Discontinuous transcription and antigenic variation in trypanosomes.
Borst P. Borst P. Annu Rev Biochem. 1986;55:701-32. doi: 10.1146/annurev.bi.55.070186.003413. Annu Rev Biochem. 1986. PMID: 2874767 Review.
Cited by
-
Improved separation of chromosome-sized DNA from Trypanosoma brucei, stock 427-60.
Van der Ploeg LH, Smith CL, Polvere RI, Gottesdiener KM. Van der Ploeg LH, et al. Nucleic Acids Res. 1989 Apr 25;17(8):3217-27. doi: 10.1093/nar/17.8.3217. Nucleic Acids Res. 1989. PMID: 2726456 Free PMC article.
-
Gottesdiener KM, Goriparthi L, Masucci JP, Van der Ploeg LH. Gottesdiener KM, et al. Mol Cell Biol. 1992 Oct;12(10):4784-95. doi: 10.1128/mcb.12.10.4784-4795.1992. Mol Cell Biol. 1992. PMID: 1406660 Free PMC article.
-
Ashby E, Paddock L, Betts HL, Liao J, Miller G, Porter A, Rollosson LM, Saada C, Tang E, Wade SJ, Hardin J, Schulz D. Ashby E, et al. mSphere. 2022 Jun 29;7(3):e0002322. doi: 10.1128/msphere.00023-22. Epub 2022 Jun 1. mSphere. 2022. PMID: 35642518 Free PMC article.
-
Urményi TP, Van der Ploeg LH. Urményi TP, et al. Nucleic Acids Res. 1995 Mar 25;23(6):1010-8. doi: 10.1093/nar/23.6.1010. Nucleic Acids Res. 1995. PMID: 7731788 Free PMC article.
-
Navarro M, Cross GA, Wirtz E. Navarro M, et al. EMBO J. 1999 Apr 15;18(8):2265-72. doi: 10.1093/emboj/18.8.2265. EMBO J. 1999. PMID: 10205179 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous