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Quality control: Genome maintenance in pluripotent stem cells - PubMed

  • ️Wed Jan 01 2014

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Quality control: Genome maintenance in pluripotent stem cells

Uri Weissbein et al. J Cell Biol. 2014.

Abstract

Pluripotent stem cells (PSCs) must maintain their proper genomic content in order to preserve appropriate self-renewal and differentiation capacities. However, their prolonged in vitro propagation, as well as the environmental culture conditions, present serious challenges to genome maintenance. Recent work has been focused on potential means to alleviate the genomic insults experienced by PSCs, and to detect them as soon as they arise, in order to prevent the detrimental consequences of these genomic aberrations on PSC application in basic research and regenerative medicine.

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Figures

Figure 1.
Figure 1.

Main challenges in the maintenance of PSC genomic integrity. Mouse and human PSCs face inherent and environmental challenges that affect how they maintain their genomic integrity. Presented are key differences between PSCs and somatic cells, which contribute to the formation of these challenges and to the way PSCs cope with them. See the text for elaboration on each of these topics.

Figure 2.
Figure 2.

Potential ways to minimize genomic insults in PSCs. The genomic insults on PSCs in culture may be alleviated by adjusting their culture conditions (i.e., the signals to which they are exposed) or by executing cell culture practices that would reduce the selection for aberrant cells. Presented are main actions that may be taken to minimize the accumulation of genetic abnormalities in PSC cultures.

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