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Nuclear distribution of RNA polymerase II and mRNA processing machinery in early mammalian embryos - PubMed

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Nuclear distribution of RNA polymerase II and mRNA processing machinery in early mammalian embryos

Irina O Bogolyubova et al. Biomed Res Int. 2014.

Abstract

Spatial distribution of components of nuclear metabolism provides a significant impact on regulation of the processes of gene expression. While distribution of the key nuclear antigens and their association with the defined nuclear domains were thoroughly traced in mammalian somatic cells, similar data for the preimplantation embryos are scanty and fragmental. However, the period of cleavage is characterized by the most drastic and dynamic nuclear reorganizations accompanying zygotic gene activation. In this minireview, we try to summarize the results of studies concerning distribution of major factors involved in RNA polymerase II-dependent transcription, pre-mRNA splicing mRNA export that have been carried out on early embryos of mammals.

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Figures

Figure 1
Figure 1

Double immunolocalization of SC35 (a), (b) and hyperphosphorylated RNA polymerase II (PolII) (a′), (b′) in mouse embryos. Fluorescence of nuclei begins to be detected only at the early 2-cell stage (line a). Association of RNAP II with SC35 domains (speckles) is observed already at this stage and is increased when ZGA finishes (line b). Bar is 10 μm, according to [24], open access.

Figure 2
Figure 2

The distribution of SR protein SC35 (a), (c), hyperphosphorylated RNA polymerase II (PolII) (b), (d) in control (a), (b), and arrested in vitro 2-cell mouse embryos (c), (d). Note the large SC35 speckles enriched in hyperphosphorylated RNA polymerase II in the nucleus of blocked embryo. Scale bar is 5 μm, according to [26], reprinted from Tissue and Cell; Bogolyubova. Transcriptional activity of nuclei in 2-cell blocked mouse embryos 2011; 43: 262-265 [26], with permission from Elsevier.

Figure 3
Figure 3

Double immunolocalization of SC35 (a), (b) and transcription factor TFIID (a′), (b′) in mouse embryos. TFIID is revealed in the nuclei at all studied stages. Colocalization of SC35 and TFIID is intensified during ZGA. Bar is 10 μm, according to [24], open access.

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References

    1. Mao YS, Zhang B, Spector DL. Biogenesis and function of nuclear bodies. Trends in Genetics. 2011;27(8):295–306. - PMC - PubMed
    1. Brahms H, Raymackers J, Union A, de Keyser F, Meheus L, Lührmann R. The C-terminal RG dipeptide repeats of the spliceosomal Sm proteins D1 and D3 contain symmetrical dimethylarginines, which form a major B-cell epitope for anti-Sm autoantibodies. The Journal of Biological Chemistry. 2000;275(22):17122–17129. - PubMed
    1. Spector DL, Fu X-D, Maniatis T. Associations between distinct pre-mRNA splicing components and the cell nucleus. The EMBO Journal. 1991;10(11):3467–3481. - PMC - PubMed
    1. Bogolyubov D, Stepanova I, Parfenov V. Universal nuclear domains of somatic and germ cells: some lessons from oocyte interchromatin granule cluster and Cajal body structure and molecular composition. BioEssays. 2009;31(4):400–409. - PubMed
    1. Spector DL, Lamond AI. Nuclear speckles. Cold Spring Harbor Perspectives in Biology. 2011;3(2)a000646 - PMC - PubMed

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