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Spontaneous antral follicle formation and metaphase II oocyte from a non-stimulated prepubertal ovarian tissue xenotransplant - PubMed

  • ️Wed Jan 01 2014

Spontaneous antral follicle formation and metaphase II oocyte from a non-stimulated prepubertal ovarian tissue xenotransplant

Laura Lotz et al. Reprod Biol Endocrinol. 2014.

Abstract

Background: Current strategies in cancer treatment have markedly increased the rates of remission and survival for cancer patients, but are often associated with subsequent sterility. While there are various options available to an adult female depending on the patient's particular situation, the only realistic option for preserving fertility in prepubertal females is to cryopreserve ovarian tissue. This is the first report of a morphologically mature oocyte collected from non-stimulated prepubertal ovarian tissue xenotransplants.

Methods: Ovarian tissue from a 6 year old patient suffering from nephroblastoma was removed and cryopreserved for fertility preservation. The frozen-thawed ovarian tissue fragments were xenotransplanted to bilaterally oophorectomized severe combined immunodeficiency (SCID) mice to assess follicle development.

Results: Antral follicle formation occurred post-xenotransplantation in a single ovarian fragment without exogenous hormone stimulation. A morphologically maturing oocyte was harvested from these follicles.

Conclusions: Prepubertal human ovarian follicles and oocytes can be matured after xenotransplantation even without exogenous hormone stimulation. These results indicate that tissue collected from prepubertal patients can support fertility in cancer survivors.

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Figures

Figure 1
Figure 1

Macroscopic view of large lumps seen at the site of the grafted prepubertal ovarian tissue Day 122 post-transplantation. Antral follicles estimated to be ~7 mm in diameter. (A) Dorsal view; (B) Side view.

Figure 2
Figure 2

One maturing oocyte was recovered from the aspirate of an antral follicle from the xenotransplanted prepubertal ovarian tissue (32x magnification).

Figure 3
Figure 3

Morphologically mature metaphase II oocyte following 22 h in in vitro culture. Oocyte measured 150 μm in diameter (32x magnification).

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