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Efficacy of ovarian tissue cryopreservation in a major European center - PubMed

Efficacy of ovarian tissue cryopreservation in a major European center

L Bastings et al. J Assist Reprod Genet. 2014 Aug.

Abstract

Purpose: To evaluate the effect of cryopreservation and thawing of ovarian tissue from oncological patients opting for fertility preservation on ovarian tissue viability.

Methods: In this prospective cohort study, the ovarian tissue viability before and after cryopreservation and thawing was measured for 25 newly diagnosed oncological patients who had their ovarian tissue cryopreserved. Outcome measures were follicle integrity (histology), follicle viability (Calcein viability assay), steroid hormone production (estradiol and progesterone production in vitro) and overall tissue viability (glucose uptake in vitro). This study was conducted at a Cryobank for storage of ovarian tissue in a university hospital.

Results: Cryopreserved/thawed ovarian tissue showed a decreased glucose uptake when compared to tissue that had not been cryopreserved. In addition, a diminished E2 and P4 production was observed after cryopreservation and thawing, despite the fact that numbers of viable follicles as determined by the Calcein viability assay were comparable. Histological examination revealed a higher percentage of degenerated follicles after cryopreservation and thawing.

Conclusions: Ovarian tissue cryopreservation and thawing impairs the viability of ovarian tissue in oncological patients opting for fertility preservation.

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Figures

Fig. 1
Fig. 1

Study design. The chain of events preceding autotransplantation of ovarian tissue according to protocols of the Cryobank Bonn. In a prospective cohort study, the viability of follicular and stromal cell compartments of the ovarian cortex was assessed directly after the tissue’s arrival at the Cryobank as well as following cryopreservation and thawing for the same patients

Fig. 2
Fig. 2

Steroid hormone production by ovarian cortex. Boxplots displaying the ovarian tissue’s E2 and P4 production before and after cryopreservation and thawing. Data are either presented per mg cortical tissue (figure a and c) or per follicle (figure b and d)

Fig. 3
Fig. 3

Glucose uptake by ovarian cortex. Boxplot displaying the ovarian tissue’s glucose uptake before and after cryopreservation and thawing per milligram tissue per hour of culture

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