Gene expression changes in progression of cervical neoplasia revealed by microarray analysis of cervical neoplastic keratinocytes - PubMed

Affiliations

• PMID: 25205602
• DOI: 10.1002/jcp.24808

Gene expression changes in progression of cervical neoplasia revealed by microarray analysis of cervical neoplastic keratinocytes

John Charles Rotondo et al. J Cell Physiol. 2015 Apr.

Abstract

To evaluate the gene expression changes involved in neoplastic progression of cervical intraepithelial neoplasia. Using microarray analysis, large-scale gene expression profile was carried out on HPV16-CIN2, HPV16-CIN3, and normal cervical keratinocytes derived from two HPV16-CIN2, two HPV-CIN3 lesions, and two corresponding normal cervical tissues, respectively. Differentially expressed genes were analyzed in normal cervical keratinocytes compared with HPV16-CIN2 keratinocytes and in HPV16-CIN2 keratinocytes compared with HPV16-CIN3 keratinocytes; 37 candidate genes with continuously increasing or decreasing expression during CIN progression were identified. One of these genes, phosphoglycerate dehydrogenase, was chosen for further characterization. Quantitative reverse transcription-polymerase chain reaction and immunohistochemical analysis confirmed that expression of phosphoglycerate dehydrogenase consistently increases during progression of CIN toward cancer. Gene expression changes occurring during CIN progression were investigated using microarray analysis, for the first time, in CIN2 and CIN3 keratinocytes naturally infected with HPV16. Phosphoglycerate dehydrogenase is likely to be associated with tumorigenesis and may be a potential prognostic marker for CIN progression.

Similar articles

• Gene expression changes during HPV-mediated carcinogenesis: a comparison between an in vitro cell model and cervical cancer.

  Wan F, Miao X, Quraishi I, Kennedy V, Creek KE, Pirisi L. Wan F, et al. Int J Cancer. 2008 Jul 1;123(1):32-40. doi: 10.1002/ijc.23463. Int J Cancer. 2008. PMID: 18398830 Free PMC article.

• HPV16 E6*II gene expression in intraepithelial cervical lesions as an indicator of neoplastic grade: a pilot study.

  Pastuszak-Lewandoska D, Bartosińska-Dyc A, Migdalska-Sęk M, Czarnecka KH, Nawrot E, Domańska D, Szyłło K, Brzeziańska E. Pastuszak-Lewandoska D, et al. Med Oncol. 2014 Mar;31(3):842. doi: 10.1007/s12032-014-0842-6. Epub 2014 Jan 17. Med Oncol. 2014. PMID: 24436016

• [Relationship among HPV16 infection and Expression of hTERT, p21waf1, Ki67 in cervical intraepithelial neoplasias and squamous cell carcinomas of cervix uteri].

  Zhao FX, Sun RF, Wang JF, Chen XW. Zhao FX, et al. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2005 Dec;19(4):370-4. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2005. PMID: 16415998 Chinese.

• Clinical implications of (epi)genetic changes in HPV-induced cervical precancerous lesions.

  Steenbergen RD, Snijders PJ, Heideman DA, Meijer CJ. Steenbergen RD, et al. Nat Rev Cancer. 2014 Jun;14(6):395-405. doi: 10.1038/nrc3728. Nat Rev Cancer. 2014. PMID: 24854082 Review.

• 'Drawing' a Molecular Portrait of CIN and Cervical Cancer: a Review of Genome-Wide Molecular Profiling Data.

  Kurmyshkina OV, Kovchur PI, Volkova TO. Kurmyshkina OV, et al. Asian Pac J Cancer Prev. 2015;16(11):4477-87. doi: 10.7314/apjcp.2015.16.11.4477. Asian Pac J Cancer Prev. 2015. PMID: 26107190 Review.

Cited by

• Low-Grade Cervical Intraepithelial Neoplasia (CIN1) Evolution: Analysis of Opportunistic Preventive Vaccination Role.

  Gardella B, Dominoni M, Pasquali MF, Melito C, Fiandrino G, Cesari S, La Verde M, Spinillo A. Gardella B, et al. Vaccines (Basel). 2023 Jan 28;11(2):284. doi: 10.3390/vaccines11020284. Vaccines (Basel). 2023. PMID: 36851162 Free PMC article.

• Human papillomavirus type 16 antagonizes IRF6 regulation of IL-1β.

  Ainouze M, Rochefort P, Parroche P, Roblot G, Tout I, Briat F, Zannetti C, Marotel M, Goutagny N, Auron P, Traverse-Glehen A, Lunel-Potencier A, Golfier F, Masson M, Robitaille A, Tommasino M, Carreira C, Walzer T, Henry T, Zanier K, Trave G, Hasan UA. Ainouze M, et al. PLoS Pathog. 2018 Aug 8;14(8):e1007158. doi: 10.1371/journal.ppat.1007158. eCollection 2018 Aug. PLoS Pathog. 2018. PMID: 30089163 Free PMC article.

• Thrombocytopenia in COVID‑19 and vaccine‑induced thrombotic thrombocytopenia.

  Geronikolou SA, Takan I, Pavlopoulou A, Mantzourani M, Chrousos GP. Geronikolou SA, et al. Int J Mol Med. 2022 Mar;49(3):35. doi: 10.3892/ijmm.2022.5090. Epub 2022 Jan 21. Int J Mol Med. 2022. PMID: 35059730 Free PMC article.

• Mathematical Modelling of Cervical Precancerous Lesion Grade Risk Scores: Linear Regression Analysis of Cellular Protein Biomarkers and Human Papillomavirus E6/E7 RNA Staining Patterns.

  Bumrungthai S, Ekalaksananan T, Kleebkaow P, Pongsawatkul K, Phatnithikul P, Jaikan J, Raumsuk P, Duangjit S, Chuenchai D, Pientong C. Bumrungthai S, et al. Diagnostics (Basel). 2023 Mar 13;13(6):1084. doi: 10.3390/diagnostics13061084. Diagnostics (Basel). 2023. PMID: 36980391 Free PMC article.

• Association of Retinoic Acid Receptor β Gene With Onset and Progression of Lichen Sclerosus-Associated Vulvar Squamous Cell Carcinoma.

  Rotondo JC, Borghi A, Selvatici R, Mazzoni E, Bononi I, Corazza M, Kussini J, Montinari E, Gafà R, Tognon M, Martini F. Rotondo JC, et al. JAMA Dermatol. 2018 Jul 1;154(7):819-823. doi: 10.1001/jamadermatol.2018.1373. JAMA Dermatol. 2018. PMID: 29898214 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Wiley

• Other Literature Sources

  • scite Smart Citations

• Medical

  • International Agency for Research on Cancer - Screening Group
  • MedlinePlus Consumer Health Information
  • MedlinePlus Health Information