Asp-286----Asn-286 in polyomavirus large T antigen relaxes the specificity of binding to the polyomavirus origin - PubMed

Asp-286----Asn-286 in polyomavirus large T antigen relaxes the specificity of binding to the polyomavirus origin

W J Tang et al. J Virol. 1989 Jan.

Abstract

We isolated revertants of a polyomavirus whose origin of DNA replication contains a point mutation in the palindrome to which large T antigen binds. Four independent second-site revertants contain an Asp-286----Asn-286 substitution in large T antigen. This mutant large T antigen activates replication of DNAs containing the mutant polyomavirus origin as well as replication of DNAs containing the wild-type origin; however, replication of DNAs with enhancer mutations is not activated by this large T antigen. The Asn-286 mutation occurs in a positively charge region of large T antigen near the location of several mutations which inactivate DNA replication. We suggest that this region of large T antigen is responsible for recognition of specific DNA sequences at the origin and that ionic forces are important for this interaction.

Similar articles

• Mutation of large T-antigen-binding site A, but not site B or C, eliminates stalling by RNA polymerase II in the intergenic region of polyomavirus DNA.

  Bertin J, Sunstrom NA, Acheson NH. Bertin J, et al. J Virol. 1993 Oct;67(10):5766-75. doi: 10.1128/JVI.67.10.5766-5775.1993. J Virol. 1993. PMID: 8396655 Free PMC article.

• Zinc-binding and protein-protein interactions mediated by the polyomavirus large T antigen zinc finger.

  Rose PE, Schaffhausen BS. Rose PE, et al. J Virol. 1995 May;69(5):2842-9. doi: 10.1128/JVI.69.5.2842-2849.1995. J Virol. 1995. PMID: 7707506 Free PMC article.

• Sequences flanking the pentanucleotide T-antigen binding sites in the polyomavirus core origin help determine selectivity of DNA replication.

  Li L, Li BL, Hock M, Wang E, Folk WR. Li L, et al. J Virol. 1995 Dec;69(12):7570-8. doi: 10.1128/JVI.69.12.7570-7578.1995. J Virol. 1995. PMID: 7494263 Free PMC article.

• Role of mouse polyomavirus late region in the control of viral DNA replication: a review.

  Iacoangeli A, Melucci-Vigo G, Risuleo G, Santi E. Iacoangeli A, et al. Biochimie. 1995;77(10):780-6. doi: 10.1016/0300-9084(96)88196-x. Biochimie. 1995. PMID: 8824775 Review.

• Simian virus 40 large T antigen: the puzzle, the pieces, and the emerging picture.

  Fanning E. Fanning E. J Virol. 1992 Mar;66(3):1289-93. doi: 10.1128/JVI.66.3.1289-1293.1992. J Virol. 1992. PMID: 1310750 Free PMC article. Review. No abstract available.

Cited by

• Determination of the origin-specific DNA-binding domain of polyomavirus large T antigen.

  Sunstrom NA, Acheson NH, Hassell JA. Sunstrom NA, et al. J Virol. 1991 Dec;65(12):6998-7003. doi: 10.1128/JVI.65.12.6998-7003.1991. J Virol. 1991. PMID: 1658390 Free PMC article.

• AP1 enhances polyomavirus DNA replication by promoting T-antigen-mediated unwinding of DNA.

  Guo W, Tang WJ, Bu X, Bermudez V, Martin M, Folk WR. Guo W, et al. J Virol. 1996 Aug;70(8):4914-8. doi: 10.1128/JVI.70.8.4914-4918.1996. J Virol. 1996. PMID: 8763994 Free PMC article.

• Genetic analysis of polyomavirus large T nuclear localization: nuclear localization is required for productive association with pRb family members.

  Howes SH, Bockus BJ, Schaffhausen BS. Howes SH, et al. J Virol. 1996 Jun;70(6):3581-8. doi: 10.1128/JVI.70.6.3581-3588.1996. J Virol. 1996. PMID: 8648692 Free PMC article.

• Inhibition of polyomavirus ori-dependent DNA replication by mSin3B.

  Xie AY, Folk WR. Xie AY, et al. J Virol. 2002 Dec;76(23):11809-18. doi: 10.1128/jvi.76.23.11809-11818.2002. J Virol. 2002. PMID: 12414923 Free PMC article.

• Cyclin-dependent kinase regulation of the replication functions of polyomavirus large T antigen.

  Li H, Bhattacharyya S, Prives C. Li H, et al. J Virol. 1997 Sep;71(9):6479-85. doi: 10.1128/JVI.71.9.6479-6485.1997. J Virol. 1997. PMID: 9261366 Free PMC article.

References

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central