Highly localized tracks of specific transcripts within interphase nuclei visualized by in situ hybridization - PubMed

Highly localized tracks of specific transcripts within interphase nuclei visualized by in situ hybridization

J B Lawrence et al. Cell. 1989.

Abstract

Use of in situ hybridization optimized for fluorescent detection of nuclear RNA has revealed a striking localization of specific viral RNAs within nuclei of cells latently infected with EBV. Several hundred kb of specific transcripts is sharply restricted to a small region of the nucleus, frequently in a curvilinear "track". Detection of nuclear RNA was evidenced by hybridization without denaturation, sensitivity to RNAase, inhibition by actinomycin D, and specificity of transcribed sequences. Results indicate that RNA "tracks" extend from an internal genome into the nuclear periphery, and that RNA transport may be coupled to transcription. Localized nRNA is apparent for other viral sequences, different lymphoblastoid cell lines, nuclei prepared by two different methods, and an abundant, nonviral transfected sequence. Implications for understanding nuclear organization and the investigation of gene expression are discussed.

Similar articles

• Nuclear RNA accumulations contain released transcripts and exhibit specific distributions with respect to Sm antigen foci.

  Lampel S, Bridger JM, Zirbel RM, Mathieu UR, Lichter P. Lampel S, et al. DNA Cell Biol. 1997 Oct;16(10):1133-42. doi: 10.1089/dna.1997.16.1133. DNA Cell Biol. 1997. PMID: 9364924

• Fluorescent detection of nuclear RNA and DNA: implications for genome organization.

  Johnson CV, Singer RH, Lawrence JB. Johnson CV, et al. Methods Cell Biol. 1991;35:73-99. Methods Cell Biol. 1991. PMID: 1723481 Review. No abstract available.

• Spatial organization of nucleic acid sequences within cells.

  Lawrence JB, Singer RH. Lawrence JB, et al. Semin Cell Biol. 1991 Apr;2(2):83-101. Semin Cell Biol. 1991. PMID: 1720683 Review.

Cited by

• Discrete nuclear domains of poly(A) RNA and their relationship to the functional organization of the nucleus.

  Carter KC, Taneja KL, Lawrence JB. Carter KC, et al. J Cell Biol. 1991 Dec;115(5):1191-202. doi: 10.1083/jcb.115.5.1191. J Cell Biol. 1991. PMID: 1720123 Free PMC article.

• Linear 2' O-Methyl RNA probes for the visualization of RNA in living cells.

  Molenaar C, Marras SA, Slats JC, Truffert JC, Lemaître M, Raap AK, Dirks RW, Tanke HJ. Molenaar C, et al. Nucleic Acids Res. 2001 Sep 1;29(17):E89-9. doi: 10.1093/nar/29.17.e89. Nucleic Acids Res. 2001. PMID: 11522845 Free PMC article.

• Deregulated expression of c-Myc in a translocation-negative plasmacytoma on extrachromosomal elements that carry IgH and myc genes.

  Wiener F, Kuschak TI, Ohno S, Mai S. Wiener F, et al. Proc Natl Acad Sci U S A. 1999 Nov 23;96(24):13967-72. doi: 10.1073/pnas.96.24.13967. Proc Natl Acad Sci U S A. 1999. PMID: 10570182 Free PMC article.

• Mechanism of mRNA transport in the nucleus.

  Vargas DY, Raj A, Marras SA, Kramer FR, Tyagi S. Vargas DY, et al. Proc Natl Acad Sci U S A. 2005 Nov 22;102(47):17008-13. doi: 10.1073/pnas.0505580102. Epub 2005 Nov 11. Proc Natl Acad Sci U S A. 2005. PMID: 16284251 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Other Literature Sources

  • The Lens - Patent Citations Database