Structure of myosin-1c tail bound to calmodulin provides insights into calcium-mediated conformational coupling - PubMed
Structure of myosin-1c tail bound to calmodulin provides insights into calcium-mediated conformational coupling
Qing Lu et al. Nat Struct Mol Biol. 2015 Jan.
Abstract
Class I myosins can sense cellular mechanical forces and function as tension-sensitive anchors or transporters. How mechanical load is transduced from the membrane-binding tail to the force-generating head in myosin-1 is unknown. Here we determined the crystal structure of the entire tail of mouse myosin-1c in complex with apocalmodulin, showing that myosin-1c adopts a stable monomer conformation suited for force transduction. The lever-arm helix and the C-terminal extended PH domain of the motor are coupled by a stable post-IQ domain bound to calmodulin in a highly unusual mode. Ca(2+) binding to calmodulin induces major conformational changes in both IQ motifs and the post-IQ domain and increases flexibility of the myosin-1c tail. Our study provides a structural blueprint for the neck and tail domains of myosin-1 and expands the target binding modes of the master Ca(2+)-signal regulator calmodulin.
Similar articles
-
Calmodulin binding to recombinant myosin-1c and myosin-1c IQ peptides.
Gillespie PG, Cyr JL. Gillespie PG, et al. BMC Biochem. 2002 Nov 26;3:31. doi: 10.1186/1471-2091-3-31. Epub 2002 Nov 26. BMC Biochem. 2002. PMID: 12453307 Free PMC article.
-
Münnich S, Taft MH, Manstein DJ. Münnich S, et al. J Mol Biol. 2014 May 15;426(10):2070-81. doi: 10.1016/j.jmb.2014.03.004. Epub 2014 Mar 14. J Mol Biol. 2014. PMID: 24636949
-
Structure, function, and regulation of myosin 1C.
Barylko B, Jung G, Albanesi JP. Barylko B, et al. Acta Biochim Pol. 2005;52(2):373-80. Epub 2005 May 31. Acta Biochim Pol. 2005. PMID: 15933767 Review.
-
Myosin-1c interacts with hair-cell receptors through its calmodulin-binding IQ domains.
Cyr JL, Dumont RA, Gillespie PG. Cyr JL, et al. J Neurosci. 2002 Apr 1;22(7):2487-95. doi: 10.1523/JNEUROSCI.22-07-02487.2002. J Neurosci. 2002. PMID: 11923413 Free PMC article.
-
Structure, dynamics and interaction with kinase targets: computer simulations of calmodulin.
Yang C, Jas GS, Kuczera K. Yang C, et al. Biochim Biophys Acta. 2004 Mar 11;1697(1-2):289-300. doi: 10.1016/j.bbapap.2003.11.032. Biochim Biophys Acta. 2004. PMID: 15023369 Review.
Cited by
-
UBE3B Is a Calmodulin-regulated, Mitochondrion-associated E3 Ubiquitin Ligase.
Braganza A, Li J, Zeng X, Yates NA, Dey NB, Andrews J, Clark J, Zamani L, Wang XH, St Croix C, O'Sullivan R, Garcia-Exposito L, Brodsky JL, Sobol RW. Braganza A, et al. J Biol Chem. 2017 Feb 10;292(6):2470-2484. doi: 10.1074/jbc.M116.766824. Epub 2016 Dec 21. J Biol Chem. 2017. PMID: 28003368 Free PMC article.
-
Arif E, Sharma P, Solanki A, Mallik L, Rathore YS, Twal WO, Nath SK, Gandhi D, Holzman LB, Ostap EM, Ashish, Nihalani D. Arif E, et al. Mol Cell Biol. 2016 May 16;36(11):1639-54. doi: 10.1128/MCB.00020-16. Print 2016 Jun 1. Mol Cell Biol. 2016. PMID: 27044863 Free PMC article.
-
How Transmembrane Inner Ear (TMIE) plays role in the auditory system: A mystery to us.
Farhadi M, Razmara E, Balali M, Hajabbas Farshchi Y, Falah M. Farhadi M, et al. J Cell Mol Med. 2021 May 13;25(13):5869-83. doi: 10.1111/jcmm.16610. Online ahead of print. J Cell Mol Med. 2021. PMID: 33987950 Free PMC article. Review.
-
iASPP contributes to cell cortex rigidity, mitotic cell rounding, and spindle positioning.
Mangon A, Salaün D, Bouali ML, Kuzmić M, Quitard S, Thuault S, Isnardon D, Audebert S, Puech PH, Verdier-Pinard P, Badache A. Mangon A, et al. J Cell Biol. 2021 Dec 6;220(12):e202012002. doi: 10.1083/jcb.202012002. Epub 2021 Oct 27. J Cell Biol. 2021. PMID: 34705028 Free PMC article.
-
Myosin-1C uses a novel phosphoinositide-dependent pathway for nuclear localization.
Nevzorov I, Sidorenko E, Wang W, Zhao H, Vartiainen MK. Nevzorov I, et al. EMBO Rep. 2018 Feb;19(2):290-304. doi: 10.15252/embr.201744296. Epub 2018 Jan 12. EMBO Rep. 2018. PMID: 29330316 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Miscellaneous