pubmed.ncbi.nlm.nih.gov

Antiprotozoal activity against Entamoeba histolytica of plants used in northeast Mexican traditional medicine. Bioactive compounds from Lippia graveolens and Ruta chalepensis - PubMed

️Wed Jan 01 2014

Antiprotozoal activity against Entamoeba histolytica of plants used in northeast Mexican traditional medicine. Bioactive compounds from Lippia graveolens and Ruta chalepensis

Ramiro Quintanilla-Licea et al. Molecules. 2014.

Abstract

Amoebiasis caused by Entamoeba histolytica is associated with high morbidity and mortality is becoming a major public health problem worldwide, especially in developing countries. Because of the side-effects and the resistance that pathogenic protozoa build against the standard antiparasitic drugs, e.g., metronidazole, much recent attention has been paid to plants used in traditional medicine around the world in order to find new antiprotozoal agents. We collected 32 plants used in Northeast Mexican traditional medicine and the methanolic extracts of these species were screened for antiprotozoal activity against E. histolytica trophozoites using in vitro tests. Only 18 extracts showed a significant inhibiting activity and among them six plant extracts showed more than 80% growth inhibition against E. histolytica at a concentration of 150 µg/mL and the IC50 values of these extracts were determined. Lippia graveolens Kunth and Ruta chalepensis Pers. showed the more significant antiprotozoal activity (91.54% and 90.50% growth inhibition at a concentration of 150 µg/mL with IC50 values of 59.14 and 60.07 µg/mL, respectively). Bioassay-guided fractionation of the methanolic extracts from these two plants afforded carvacrol (1) and chalepensin (2), respectively, as bioactive compounds with antiprotozoal activity.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

**Figure 1**
Structure of carvacrol (1) and chalepensin (2).

Cited by

Antiprotozoal Activity against Entamoeba histolytica of Flavonoids Isolated from Lippia graveolens Kunth.
Quintanilla-Licea R, Vargas-Villarreal J, Verde-Star MJ, Rivas-Galindo VM, Torres-Hernández ÁD. Quintanilla-Licea R, et al. Molecules. 2020 May 26;25(11):2464. doi: 10.3390/molecules25112464. Molecules. 2020. PMID: 32466359 Free PMC article.
Cytotoxic, Anti-Hemolytic, and Antioxidant Activities of Ruta chalepensis L. (Rutaceae) Extract, Fractions, and Isolated Compounds.
Elizondo-Luévano JH, Rodríguez-Garza NE, Bazaldúa-Rodríguez AF, Romo-Sáenz CI, Tamez-Guerra P, Verde-Star MJ, Gomez-Flores R, Quintanilla-Licea R. Elizondo-Luévano JH, et al. Plants (Basel). 2023 Jun 2;12(11):2203. doi: 10.3390/plants12112203. Plants (Basel). 2023. PMID: 37299182 Free PMC article.
Ruta Essential Oils: Composition and Bioactivities.
Nahar L, El-Seedi HR, Khalifa SAM, Mohammadhosseini M, Sarker SD. Nahar L, et al. Molecules. 2021 Aug 6;26(16):4766. doi: 10.3390/molecules26164766. Molecules. 2021. PMID: 34443352 Free PMC article. Review.
Antioxidant and Antibacterial Activity of Mexican Oregano Essential Oil, Extracted from Plants Occurring Naturally in Semiarid Areas and Cultivated in the Field and Greenhouse in Northern Mexico.
Marin-Tinoco RI, Ortega-Ramírez AT, Esteban-Mendez M, Silva-Marrufo O, Barragan-Ledesma LE, Valenzuela-Núñez LM, Briceño-Contreras EA, Sariñana-Navarrete MA, Camacho-Luis A, Navarrete-Molina C. Marin-Tinoco RI, et al. Molecules. 2023 Sep 9;28(18):6547. doi: 10.3390/molecules28186547. Molecules. 2023. PMID: 37764323 Free PMC article.
Chalepin and Chalepensin: Occurrence, Biosynthesis and Therapeutic Potential.
Nahar L, Al-Majmaie S, Al-Groshi A, Rasul A, Sarker SD. Nahar L, et al. Molecules. 2021 Mar 14;26(6):1609. doi: 10.3390/molecules26061609. Molecules. 2021. PMID: 33799365 Free PMC article. Review.

References

1. Kirmizibekmez H., Atay I., Kaiser M., Brun R., Cartagena M.M., Carballeira N.M., Yesilada E., Tasdemir D. Antiprotozoal activity of Melampyrum arvense and its metabolites. Phytother. Res. 2011;25:142–146. doi: 10.1002/ptr.3233. - DOI - PubMed
1. Martinez P.A., Petersen C.A. Chronic infection by Leishmania amazonensis mediated through MAPK ERK mechanisms. Immunol. Res. 2014;59:153–165. doi: 10.1007/s12026-014-8535-y. - DOI - PMC - PubMed
1. Sosoniuk E., Vallejos G., Kenawy H., Gaboriaud C., Thielens N., Fujita T., Schwaeble W., Ferreira A., Valck C. Trypanozoma cruzi calreticulin inhibits the complement lectin pathway activation by direct interaction with L-Ficolin. Mol. Immunol. 2014;60:80–85. doi: 10.1016/j.molimm.2014.03.014. - DOI - PubMed
1. Hotez P.J., Woc-Colburn L., Bottazzi M.E. Neglected tropical diseases in Central America and Panama: Review of their prevalence, populations at risk and impact on regional development. Int. J. Parasitol. 2014;44:597–603. doi: 10.1016/j.ijpara.2014.04.001. - DOI - PubMed
1. Zerpa-Larrauri R., Náquira-Velarde C., Espinoza Y. A new vision of Entamoeba histolytica. Rev. Peru. Med. Exp. Salud Publica. 2007;24:190–192.

Antiprotozoal activity against Entamoeba histolytica of plants used in northeast Mexican traditional medicine. Bioactive compounds from Lippia graveolens and Ruta chalepensis - PubMed