pubmed.ncbi.nlm.nih.gov

Transcriptome sequencing in a Tibetan barley landrace with high resistance to powdery mildew - PubMed

Transcriptome sequencing in a Tibetan barley landrace with high resistance to powdery mildew

Xing-Quan Zeng et al. ScientificWorldJournal. 2014.

Abstract

Hulless barley is an important cereal crop worldwide, especially in Tibet of China. However, this crop is usually susceptible to powdery mildew caused by Blumeria graminis f. sp. hordei. In this study, we aimed to understand the functions and pathways of genes involved in the disease resistance by transcriptome sequencing of a Tibetan barley landrace with high resistance to powdery mildew. A total of 831 significant differentially expressed genes were found in the infected seedlings, covering 19 functions. Either "cell," "cell part," and "extracellular region" in the cellular component category or "binding" and "catalytic" in the category of molecular function as well as "metabolic process" and "cellular process" in the biological process category together demonstrated that these functions may be involved in the resistance to powdery mildew of the hulless barley. In addition, 330 KEGG pathways were found using BLASTx with an E-value cut-off of <10(-5). Among them, three pathways, namely, "photosynthesis," "plant-pathogen interaction," and "photosynthesis-antenna proteins" had significant matches in the database. Significant expressions of the three pathways were detected at 24 h, 48 h, and 96 h after infection, respectively. These results indicated a complex process of barley response to powdery mildew infection.

PubMed Disclaimer

Figures

Figure 1
Figure 1

Mapping reads (exon, intron, and intergene) coverage of A (C0, TR130348).

Figure 2
Figure 2

Distributed density of gene global expression of each sample.

Figure 3
Figure 3

Euclidean distance was used to establish the distance of expression between A (C0, TR130348) and B (C24, TR130349).

Figure 4
Figure 4

Histogram presentation of gene ontology classification between A (C0, TR130348) and B (C24, TR130349). The results are summarized in three main categories: biological process, cellular component, and molecular function. The right y-axis indicates the number of genes in a category. The left y-axis indicates the percentage of a specific category of genes in that main category.

Similar articles

Cited by

References

    1. Bhatty R. S. β-glucan and flour yield of hull-less barley. Cereal Chemistry. 1999;76(2):314–315. doi: 10.1094/cchem.1999.76.2.314. - DOI
    1. McDonald B. A., Linde C. The population genetics of plant pathogens and breeding strategies for durable resistance. Euphytica. 2002;124(2):163–180. doi: 10.1023/a:1015678432355. - DOI
    1. Fischbeck G., Jahoor A. The transfer of genes for mildew resistance from Hordeum spontaneum . In: Jørgensen J. H., editor. Integrated Control of Cereal Mildews: Virulence Patterns and Their Change. Roskilde, Denmark: Risø National Laboratory; 1991. pp. 247–255. http://agris.fao.org/agris-search/search.do?recordID=DK9420942.
    1. Jørgensen J. H., Wolfe P. M. Genetics of powdery mildew resistance in barley. Critical Reviews in Plant Sciences. 1994;13(1):97–119. doi: 10.1080/07352689409701910. - DOI
    1. Helms Jørgensen J., Jensen H. P. Powdery mildew resistance in barley landrace material. I. Screening for resistance. Euphytica. 1997;97(2):227–233. doi: 10.1023/A:1003032424968. - DOI

Publication types

MeSH terms

LinkOut - more resources