Partition locus-based classification of selected plasmids in Klebsiella pneumoniae, Escherichia coli and Salmonella enterica spp.: an additional tool - PubMed

Partition locus-based classification of selected plasmids in Klebsiella pneumoniae, Escherichia coli and Salmonella enterica spp.: an additional tool

A Bousquet et al. J Microbiol Methods. 2015 Mar.

Free article

Abstract

The dissemination of antimicrobial resistance genes in Enterobacteriaceae has been largely attributed to plasmids, circular DNA molecules capable of autonomous replication. Whereas high-copy-number plasmids primarily rely on passive diffusion for plasmid maintenance, low-copy-number plasmids utilize so-called partition (par) systems. Plasmid partition relies on three structures, i.e. a centromere like DNA site, a centromere-binding protein and an ATPase or a GTPase motor protein for plasmid positioning. Identification and classification of plasmids is essential for tracing plasmids conferring drug resistance. PCR-based replicon typing is currently the standard method for plasmid identification but there are new classification schemes, especially the relaxase gene typing (PRaseT). Here we developed a multiplex PCR set targeting par loci found on the plasmids most frequently encountered in Enterobacteriaceae. This method, called "plasmid partition gene typing" (PAR-T), was validated with 136 transconjugants or transformants harboring various replicon types. The method was tested with 30 multidrug-resistant clinical isolates including Escherichia coli, Klebsiella pneumoniae and Salmonella enterica subsp. enterica carrying 1-4 replicons; all replicons were tested in parallel with PRaseT for comparison. Six multiplex PCRs and one simplex PCR including 18 pairs of primers recognized plasmids of groups IncA/C, FIA, FIB, FIC, FIIk, FII, HI1, HI2, I1, L/M, N, X. Our set of multiplex PCRs showed high specificity for the classification of resistance plasmids except for IncX replicons.

Keywords: Incompatibility group; Partition system; Plasmid.

Similar articles

• Targeting relaxase genes for classification of the predominant plasmids in Enterobacteriaceae.

  Compain F, Poisson A, Le Hello S, Branger C, Weill FX, Arlet G, Decré D. Compain F, et al. Int J Med Microbiol. 2014 May;304(3-4):236-42. doi: 10.1016/j.ijmm.2013.09.009. Epub 2013 Oct 19. Int J Med Microbiol. 2014. PMID: 24342269

• Identification of plasmids by PCR-based replicon typing.

  Carattoli A, Bertini A, Villa L, Falbo V, Hopkins KL, Threlfall EJ. Carattoli A, et al. J Microbiol Methods. 2005 Dec;63(3):219-28. doi: 10.1016/j.mimet.2005.03.018. Epub 2005 Jun 2. J Microbiol Methods. 2005. PMID: 15935499

• Conjugative transferability of the A/C plasmids from Salmonella enterica isolates that possess or lack bla(CMY) in the A/C plasmid backbone.

  Poole TL, Edrington TS, Brichta-Harhay DM, Carattoli A, Anderson RC, Nisbet DJ. Poole TL, et al. Foodborne Pathog Dis. 2009 Dec;6(10):1185-94. doi: 10.1089/fpd.2009.0316. Foodborne Pathog Dis. 2009. PMID: 19743926

• Plasmid Localization and Partition in Enterobacteriaceae.

  Bouet JY, Funnell BE. Bouet JY, et al. EcoSal Plus. 2019 Jun;8(2):10.1128/ecosalplus.ESP-0003-2019. doi: 10.1128/ecosalplus.ESP-0003-2019. EcoSal Plus. 2019. PMID: 31187729 Free PMC article. Review.

• Structural biology of plasmid partition: uncovering the molecular mechanisms of DNA segregation.

  Schumacher MA. Schumacher MA. Biochem J. 2008 May 15;412(1):1-18. doi: 10.1042/BJ20080359. Biochem J. 2008. PMID: 18426389 Review.

Cited by

• Mobile Genetic Elements Associated with Antimicrobial Resistance.

  Partridge SR, Kwong SM, Firth N, Jensen SO. Partridge SR, et al. Clin Microbiol Rev. 2018 Aug 1;31(4):e00088-17. doi: 10.1128/CMR.00088-17. Print 2018 Oct. Clin Microbiol Rev. 2018. PMID: 30068738 Free PMC article. Review.

• Complete sequence of classic F-type plasmid pRK100 shows unique conservation over time and geographic location.

  Starčič Erjavec M, Jeseničnik K, Elam LP, Kastrin A, Predojević L, Sysoeva TA. Starčič Erjavec M, et al. Plasmid. 2022 Jan-Mar;119-120:102618. doi: 10.1016/j.plasmid.2022.102618. Epub 2022 Jan 22. Plasmid. 2022. PMID: 35077724 Free PMC article.

• First Description of IncX3 Plasmids Carrying blaOXA-181 in Escherichia coli Clinical Isolates in Burkina Faso.

  Ouédraogo AS, Compain F, Sanou M, Aberkane S, Bouzinbi N, Hide M, Sangaré L, Ouédraogo-Traoré R, Jean-Pierre H, Vendrell J, Solassol J, Decré D, Godreuil S. Ouédraogo AS, et al. Antimicrob Agents Chemother. 2016 Apr 22;60(5):3240-2. doi: 10.1128/AAC.00147-16. Print 2016 May. Antimicrob Agents Chemother. 2016. PMID: 26883710 Free PMC article. No abstract available.

• Plasmid Classification in an Era of Whole-Genome Sequencing: Application in Studies of Antibiotic Resistance Epidemiology.

  Orlek A, Stoesser N, Anjum MF, Doumith M, Ellington MJ, Peto T, Crook D, Woodford N, Walker AS, Phan H, Sheppard AE. Orlek A, et al. Front Microbiol. 2017 Feb 9;8:182. doi: 10.3389/fmicb.2017.00182. eCollection 2017. Front Microbiol. 2017. PMID: 28232822 Free PMC article.

• Genomics and Virulence of Klebsiella pneumoniae Kpnu95 ST1412 Harboring a Novel Incf Plasmid Encoding Blactx-M-15 and Qnrs1 Causing Community Urinary Tract Infection.

  Gancz A, Kondratyeva K, Cohen-Eli D, Navon-Venezia S. Gancz A, et al. Microorganisms. 2021 May 10;9(5):1022. doi: 10.3390/microorganisms9051022. Microorganisms. 2021. PMID: 34068663 Free PMC article.

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • ClinicalKey
  • Elsevier Science
  • HAL archives ouvertes

• Other Literature Sources

  • The Lens - Patent Citations Database

• Miscellaneous

  • NCI CPTAC Assay Portal