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Human and murine erythropoiesis - PubMed

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Review

Human and murine erythropoiesis

Xiuli An et al. Curr Opin Hematol. 2015 May.

Abstract

Purpose of review: Research into the fundamental mechanisms of erythropoiesis has provided critical insights into inherited and acquired disorders of the erythrocyte. Studies of human erythropoiesis have primarily utilized in-vitro systems, whereas murine models have provided insights from in-vivo studies. This report reviews recent insights into human and murine erythropoiesis gained from transcriptome-based analyses.

Recent findings: The availability of high-throughput genomic methodologies has allowed attainment of detailed gene expression data from cells at varying developmental and differentiation stages of erythropoiesis. Transcriptome analyses of human and murine reveal both stage and species-specific similarities and differences across terminal erythroid differentiation. Erythroid-specific long noncoding RNAs exhibit poor sequence conservation between human and mouse. Genome-wide analyses of alternative splicing reveal that complex, dynamic, stage-specific programs of alternative splicing program are utilized during terminal erythroid differentiation. Transcriptome data provide a significant resource for understanding mechanisms of normal and perturbed erythropoiesis. Understanding these processes will provide innovative strategies to detect, diagnose, prevent, and treat hematologic disease.

Summary: Understanding the shared and different mechanisms controlling human and murine erythropoiesis will allow investigators to leverage the best model system to provide insights in normal and perturbed erythropoiesis.

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Conflict of interest statement

Conflicts of interest

None of the authors have any conflicts of interest to report.

Figures

FIGURE 1
FIGURE 1

Human and murine erythroid cells have distinct expression profiles at the SLC2A1 locus. RNA obtained from human and murine erythroid cells at different stages of erythroid differentiation underwent RNA-seq. Integrated genome browser views of normalized mapped RNA-seq read coverage at the SLC2A1 locus in human and mouse. There is robust expression in human cells at all stages of erythroid differentiation, whereas there is no expression in murine erythroid cells at any stage.

FIGURE 2
FIGURE 2

Transcriptome analyses. Human and murine erythroid cells have distinct expression profiles. RNA obtained from human and murine erythroid cells at different stages of erythroid differentiation underwent RNA-seq. The figure demonstrates graphical depiction of a pattern of gene expression levels of a cluster of 318 differentially expressed genes in human and mouse cells. Bioinformatic analyses predict this cluster is related to protein processing.

FIGURE 3
FIGURE 3

Stage-specific premature chain termination (PCT) splicing events in human erythroblasts. (a) Transcripts in which PCT isoforms are more abundant in late erythroblasts because of upregulation of PCT-exons. (b) Transcripts in which PCT transcripts are relatively more abundant in early erythroblasts because of exon skipping events that alter translational reading frame. Gene names and differentiation stage are indicated above each lane, whereas calculated percent spliced in (PSI) values are shown below each lane. Arrowheads indicate PCR bands representing PCT isoforms. * indicates genes for which PCR results indicate larger splicing changes than were predicted bioinformatically. Reprinted with permission from [32▪▪].

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