pubmed.ncbi.nlm.nih.gov

The role of silicon in enhancing resistance to bacterial blight of hydroponic- and soil-cultured rice - PubMed

  • ️Fri Jan 01 2016

The role of silicon in enhancing resistance to bacterial blight of hydroponic- and soil-cultured rice

Alin Song et al. Sci Rep. 2016.

Abstract

Here we report for the first time that bacterial blight of rice can be alleviated by silicon (Si) added. In both inoculated and uninoculated plants, shoot dry weight was significantly higher in the +Si plants than in the -Si plants. A soil-cultured trial showed that disease severity was 24.3% lower in the Si-amended plants than in the non-Si-amended plants. Plants that were switched from -Si to +Si nutrient solution and simultaneously inoculated with Xoo also exhibited the same high resistance to bacterial blight as the plants that were treated continuously with Si, with control efficiencies of 52.8 and 62.9%, respectively. Moreover, total concentrations of soluble phenolics and lignin in rice leaves were significantly higher in the +Si plants than in the -Si plants. Polyphenoloxidase (PPO) and phenylalanine ammonia-lyase (PAL) activities in rice leaves were observed to be higher in the +Si plants than in the -Si plants. The expression levels of Os03g0109600, Prla, Rcht2 and Lox2osPil, were also higher in +Si plants than in -Si plants post-inoculation during the experimental time. Addition of Si resulted in increased Pal transcription, and inhibited CatA and Os03g0126000 expression in the earlier and later stages of bacterial inoculation, respectively.

PubMed Disclaimer

Figures

Figure 1
Figure 1

Dry weight of shoots and roots of rice seedlings non-inoculated (−P) or inoculated with Xoo (+P) under different silicon treatments in hydroponic (A) and soil-cultured (B) experiments. Bars are mean value of three replicates. Vertical lines represent standard deviations.

Figure 2
Figure 2

Silicon concentration of shoots and roots of rice seedlings non-inoculated (−P) or inoculated with Xoo (+P) under different silicon treatments in hydroponic (A) and soil-cultured (B) experiments. Bars are mean value of three replicates. Vertical lines represent standard deviations.

Figure 3
Figure 3

Changes in activities of phenylalanine ammonia-lyase (PAL) (A) and polyphenoloxidase (PPO) (B) in leaves of rice seedlings amended with silicon (+Si) or not (−Si) post-inoculation with Xoo. The values are means of three replications. Vertical lines represent standard deviations. Asterisks denote significant difference at P < 0.05 between +Si and −Si treatments at a same time-point according to Student’s t-test.

Figure 4
Figure 4

Changes in concentrations of total soluble phenolics (A) and lignin (B) in leaves of rice seedlings amended with silicon (+Si) or not (−Si) post-inoculation with Xoo. The values are means of three replications. Vertical lines represent standard deviations. Asterisks denote significant difference at P < 0.05 between +Si and −Si treatments at a same time-point according to Student’s t-test.

Figure 5
Figure 5

Relative expression levels of (A) Os03g0109600, (B) Os03g0126000, (C) Pal, (D) Prla, (E) Rcht2, (F) Lox2osPil, and (G) CatA genes in leaves of rice seedlings amended with silicon (+Si) or not (−Si) post-inoculation with Xoo. The values are means of three replications. Vertical lines represent standard deviations. Asterisks denote significant difference at P < 0.05 between +Si and −Si treatments at a same time-point according to Student’s t-test.

Similar articles

Cited by

References

    1. Geetha N. E., Amruthesh K. N., Sharathchandra R. G. & Shetty H. S. Resistance to downy mildew in pearl millet is associated with increased phenylalanine ammonia-lyase activity. Funct. Plant Biol. 32, 267–275 (2005). - PubMed
    1. Umesha S. Phenylalanine ammonia lyase activity in tomato seedlings and its relationship to bacterial canker disease resistance. Phytoparasitica. 34, 68–71 (2006).
    1. Das K. K., Panda D., Nagaraju M., Sharma S. G. & Sarkar R. K. Antioxidant enzymes and aldehyde releasing capacity of rice cultivars (Oryzae sativa L.) as determinants of anaerobic seedling establishment capacity. Bulg J. Plant Physiol. 30, 34–44 (2004).
    1. Zhu Y. J. et al.. Systemic acquired resistance induced by BTH in papaya. Physiol. Molec. Plant Pathol. 63, 237–248 (2003).
    1. Shrestha C. L., Oña I., Muthukrishnan S. & Mew T. W. Chitinase levels in rice cultivars correlate with resistance to the sheath blight pathogen Rhizoctonia solani. Eur. J. Plant Pathology. 120, 69–77 (2008).

Publication types

MeSH terms

Substances

LinkOut - more resources