Induction and repression of the Drosophila Sgs-3 glue gene are mediated by distinct sequences in the proximal promoter - PubMed
Induction and repression of the Drosophila Sgs-3 glue gene are mediated by distinct sequences in the proximal promoter
M Martin et al. EMBO J. 1989 Feb.
Abstract
The normal developmental expression of the Drosophila salivary gland secretion protein gene Sgs-3 requires the interaction of a distal and proximal regulatory element. A deletion/replacement analysis of the proximal promoter in stably transformed lines shows that induction of an Sgs-3/Adh fusion gene is normal if sequences from +10 to -50 are replaced by those of the hsp70 gene. Sequences between -98 and -50 are necessary for this expression but there is internal redundancy within this region as two distinct upstream sequences of 18 and 22 bp respectively are sufficient for stage- and tissue-specific expression, albeit at reduced levels. A point mutation at -53 eliminates the ecdysone-mediated repression of the Sgs-3 promoter at pupariation. We report mosaicisms of expression within the salivary gland for a number of stably transformed lines.
Similar articles
-
Kress H, Swida U. Kress H, et al. Naturwissenschaften. 1990 Jul;77(7):317-24. doi: 10.1007/BF01138385. Naturwissenschaften. 1990. PMID: 2205808 Review.
-
Lehmann M, Korge G. Lehmann M, et al. EMBO J. 1995 Feb 15;14(4):716-26. doi: 10.1002/j.1460-2075.1995.tb07050.x. EMBO J. 1995. PMID: 7882975 Free PMC article.
-
Hofmann A, Garfinkel MD, Meyerowitz EM. Hofmann A, et al. Mol Cell Biol. 1991 Jun;11(6):2971-9. doi: 10.1128/mcb.11.6.2971-2979.1991. Mol Cell Biol. 1991. PMID: 1903838 Free PMC article.
-
Cooperative enhancement at the Drosophila Sgs-3 locus.
Roark M, Raghavan KV, Todo T, Mayeda CA, Meyerowitz EM. Roark M, et al. Dev Biol. 1990 May;139(1):121-33. doi: 10.1016/0012-1606(90)90283-o. Dev Biol. 1990. PMID: 2328832
-
Regulatory elements and interactions in the Drosophila 68C glue gene cluster.
Martin M, Mettling C, Giangrande A, Ruiz C, Richards G. Martin M, et al. Dev Genet. 1989;10(3):189-97. doi: 10.1002/dvg.1020100308. Dev Genet. 1989. PMID: 2500283 Review.
Cited by
-
Todo T, Roark M, Raghavan KV, Mayeda C, Meyerowitz E. Todo T, et al. Mol Cell Biol. 1990 Nov;10(11):5991-6002. doi: 10.1128/mcb.10.11.5991-6002.1990. Mol Cell Biol. 1990. PMID: 2122237 Free PMC article.
-
Kress H, Swida U. Kress H, et al. Naturwissenschaften. 1990 Jul;77(7):317-24. doi: 10.1007/BF01138385. Naturwissenschaften. 1990. PMID: 2205808 Review.
-
Wallrath LL, Burnett JB, Friedman TB. Wallrath LL, et al. Mol Cell Biol. 1990 Oct;10(10):5114-27. doi: 10.1128/mcb.10.10.5114-5127.1990. Mol Cell Biol. 1990. PMID: 2118989 Free PMC article.
-
Giangrande A, Mettling C, Martin M, Ruiz C, Richards G. Giangrande A, et al. EMBO J. 1989 Nov;8(11):3459-66. doi: 10.1002/j.1460-2075.1989.tb08510.x. EMBO J. 1989. PMID: 2583107 Free PMC article.
-
Mach V, Ohno K, Kokubo H, Suzuki Y. Mach V, et al. Nucleic Acids Res. 1996 Jun 15;24(12):2387-94. doi: 10.1093/nar/24.12.2387. Nucleic Acids Res. 1996. PMID: 8710511 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases