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Detection of a Yersinia pestis gene homologue in rodent samples - PubMed

  • ️Fri Jan 01 2016

Detection of a Yersinia pestis gene homologue in rodent samples

Timothy A Giles et al. PeerJ. 2016.

Abstract

A homologue to a widely used genetic marker, pla, for Yersinia pestis has been identified in tissue samples of two species of rat (Rattus rattus and Rattus norvegicus) and of mice (Mus musculus and Apodemus sylvaticus) using a microarray based platform to screen for zoonotic pathogens of interest. Samples were from urban locations in the UK (Liverpool) and Canada (Vancouver). The results indicate the presence of an unknown bacterium that shares a homologue for the pla gene of Yersinia pestis, so caution should be taken when using this gene as a diagnostic marker.

Keywords: Microarray; Rodents; Screening; Yersinia pestis.

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Conflict of interest statement

The authors declare there are no competing interests.

Figures

Figure 1
Figure 1. Sequence showing how extracted DNA is used for sequence enrichment capture (A) and microarray hybridisation (B).

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References

    1. Biggins DE, Kosoy MY. Influences of introduced plague on North American mammals: implications from ecology of plague in Asia. Journal of Mammalogy. 2001;82:906–916. doi: 10.1644/1545-1542(2001)082<0906:IOIPON>2.0.CO;2. - DOI
    1. Broekhuijsen M, Larsson P, Johansson A, Byström M, Eriksson U, Larsson E, Prior RG, Sjöstedt A, Titball FW, Forsman M. Genome-wide DNA microarray analysis of Francisella tularensis strains demonstrates extensive genetic conservation within the species but identifies regions that are unique to the highly virulent F. tularensis subsp. tularensis. Journal of Clinical Microbiology. 2003;41:2924–2931. doi: 10.1128/JCM.41.7.2924-2931.2003. - DOI - PMC - PubMed
    1. Eisen RJ, Gage KL. Adaptive strategies of Yersinia pestis to persist during inter-epizootic and epizootic periods. Veterinary Research. 2009;40(2):01. doi: 10.1051/vetres:2008039. - DOI - PMC - PubMed
    1. Eppinger M, Guo Z, Sebastian Y, Song Y, Lindler LE, Yang R, Ravel J. Draft genome sequences of Yersinia pestis isolates from natural foci of endemic plague in China. Journal of Bacteriology. 2009;191:7628–7629. doi: 10.1128/JB.01227-09. - DOI - PMC - PubMed
    1. Eppinger M, Worsham PL, Nikolich MP, Riley DR, Sebastian Y, Mou S, Achtman M, Lindler LE, Ravel J. Genome sequence of the deep-rooted yersinia pestis strain angola reveals new insights into te evolution and pangenome of the plague bacterium. Journal of Bacteriology. 2010;192:1685–1699. doi: 10.1128/JB.01518-09. - DOI - PMC - PubMed

Grants and funding

This research has received funding from the European Union Seventh Framework Programme (FP7/2007-2013) under grant agreement no 222633. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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