Micro-C XL: assaying chromosome conformation from the nucleosome to the entire genome - PubMed

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• PMID: 27723753
• DOI: 10.1038/nmeth.4025

Micro-C XL: assaying chromosome conformation from the nucleosome to the entire genome

Tsung-Han S Hsieh et al. Nat Methods. 2016 Dec.

Abstract

We present Micro-C XL, an improved method for analysis of chromosome folding at mononucleosome resolution. Using long crosslinkers and isolation of insoluble chromatin, Micro-C XL increases signal-to-noise ratio. Micro-C XL maps of budding and fission yeast genomes capture both short-range chromosome fiber features such as chromosomally interacting domains and higher order features such as centromere clustering. Micro-C XL provides a single assay to interrogate chromosome folding at length scales from the nucleosome to the full genome.

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