Association of mRNA expression of toll-like receptor 2 and 3 with hepatitis B viral load in chronic hepatitis, cirrhosis, and hepatocellular carcinoma - PubMed

Affiliations

• PMID: 27769109
• DOI: 10.1002/jmv.24719

Association of mRNA expression of toll-like receptor 2 and 3 with hepatitis B viral load in chronic hepatitis, cirrhosis, and hepatocellular carcinoma

Kangkana Kataki et al. J Med Virol. 2017 Jun.

Abstract

During Hepatitis B virus infection, the pathogen sensors Toll-like receptors (TLRs) play a role in innate immunity system. The study aimed to investigate mRNA expression levels of TLR2 and TLR3 in Hepatitis B virus (HBV) mediated chronic hepatitis B (CHB), cirrhosis (CIRR), and hepatocellular carcinoma (HCC), and to correlate viral load with severity of these diseases and expression of TLRs. A total of 180 HBV DNA positive samples were selected for the study. HVB-DNA was detected by multiplex PCR. Viral load estimation was done by using the Ampisure HBV Quantitative kit as per manufacture instructions. Expression levels of TLR2 and TLR3 were determined by real time PCR. The viral load was estimated to be 6.64log₁₀ IU/ml in CHB, 4.88log₁₀ IU/ml in CIRR, and 4.86log₁₀ IU/ml in HCC. No significant association of viral load was found with increasing age. Upregulation of TLR2 expression in CHB when individually compared with CIRR and HCC was found to be statistically significant. Downregulation of TLR3 expressions in CIRR when compared to both CHB and HCC individually were found to be statistically significant. No significant effect of viral load on the expression of TLR2 and 3 were found. With severity of the disease from CHB to HCC, the HBV load decreases. The study suggests the possibility of HBV interacting with signalling of both analysed TLR receptors which partially explains the induction of immune tolerance pathways by Hepatitis B virus. J. Med. Virol. 89:1008-1014, 2017. © 2017 Wiley Periodicals, Inc.

Keywords: CHB; CIRR; HCC; Hepatitis B virus; innate immunity; toll-like receptor.

Similar articles

• Relationship between expression of toll-like receptors 2/4 in dendritic cells and chronic hepatitis B virus infection.

  Lu X, Xu Q, Bu X, Ma X, Zhang F, Deng Q, Zhang Y, Ding J. Lu X, et al. Int J Clin Exp Pathol. 2014 Aug 15;7(9):6048-55. eCollection 2014. Int J Clin Exp Pathol. 2014. PMID: 25337250 Free PMC article.

• Correlation of circulating TLR2/4 expression with CD3+/4+/8+ T cells and Treg cells in HBV-related liver cirrhosis.

  Lian JQ, Wang XQ, Zhang Y, Huang CX, Bai XF. Lian JQ, et al. Viral Immunol. 2009 Oct;22(5):301-8. doi: 10.1089/vim.2009.0039. Viral Immunol. 2009. PMID: 19811087

• Can Toll-Like Receptor (TLR) 2 be considered as a new target for immunotherapy against hepatitis B infection?

  Bagheri V, Askari A, Arababadi MK, Kennedy D. Bagheri V, et al. Hum Immunol. 2014 Jun;75(6):549-54. doi: 10.1016/j.humimm.2014.02.018. Epub 2014 Feb 12. Hum Immunol. 2014. PMID: 24530748 Review.

• TLR3 and TLR4 SNP variants in the liver disease resulting from hepatitis B virus and hepatitis C virus infection.

  Sghaier I, Zidi S, Mouelhi L, Ghazoueni E, Brochot E, Almawi WY, Loueslati BY. Sghaier I, et al. Br J Biomed Sci. 2019 Jan;76(1):35-41. doi: 10.1080/09674845.2018.1547179. Epub 2018 Dec 3. Br J Biomed Sci. 2019. PMID: 30421643

• What can we learn from hepatitis B virus clinical cohorts?

  Lin CL, Tseng TC, Kao JH. Lin CL, et al. Liver Int. 2015 Jan;35 Suppl 1:91-9. doi: 10.1111/liv.12716. Liver Int. 2015. PMID: 25529093 Review.

Cited by

• In situ self-assembly of Au-antimiR-155 nanocomplexes mediates TLR3-dependent apoptosis in hepatocellular carcinoma cells.

  Yin L, Cai W, Liang Y, Yao J, Wang X, Shen J. Yin L, et al. Aging (Albany NY). 2020 Nov 5;13(1):241-261. doi: 10.18632/aging.103799. Epub 2020 Nov 5. Aging (Albany NY). 2020. PMID: 33173017 Free PMC article.

• Polymorphisms in the Toll-like receptor 3 (TLR3) gene are associated with the natural course of hepatitis B virus infection in Caucasian population.

  Fischer J, Koukoulioti E, Schott E, Fülöp B, Heyne R, Berg T, van Bömmel F. Fischer J, et al. Sci Rep. 2018 Aug 24;8(1):12737. doi: 10.1038/s41598-018-31065-6. Sci Rep. 2018. PMID: 30143709 Free PMC article.

• Toll-like receptors, long non-coding RNA NEAT1, and RIG-I expression are associated with HBeAg-positive chronic hepatitis B patients in the active phase.

  Zeng Y, Wu W, Fu Y, Chen S, Chen T, Yang B, Ou Q. Zeng Y, et al. J Clin Lab Anal. 2019 Jun;33(5):e22886. doi: 10.1002/jcla.22886. Epub 2019 Mar 29. J Clin Lab Anal. 2019. PMID: 30924966 Free PMC article.

• Identification of serum β-catenin as a biomarker in patients with HBV-related liver diseases.

  Duan L, Yang Q, Yang J, Hu Q, Wang B, Li P, Chen W. Duan L, et al. J Transl Med. 2018 Sep 29;16(1):265. doi: 10.1186/s12967-018-1645-x. J Transl Med. 2018. PMID: 30268125 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Ovid Technologies, Inc.
  • Wiley

• Other Literature Sources

  • scite Smart Citations

• Medical

  • MedlinePlus Health Information