The rev (trs/art) protein of human immunodeficiency virus type 1 affects viral mRNA and protein expression via a cis-acting sequence in the env region - PubMed

The rev (trs/art) protein of human immunodeficiency virus type 1 affects viral mRNA and protein expression via a cis-acting sequence in the env region

M Hadzopoulou-Cladaras et al. J Virol. 1989 Mar.

Abstract

The study of expression of several human immunodeficiency virus type 1 proviral mutants in human cells in the presence or absence of rev (trs/art) protein reveals that rev increases the levels of unspliced and env mRNA and the accumulated structural viral proteins. rev protein produced from appropriate expression vectors fully complements the rev-defective mutants. rev requires the presence of a specific cis-acting sequence for its function. This rev-responsive element sequence has been localized within a 520 base-pair fragment in the env region of human immunodeficiency virus type 1. gag and env expression is coordinately regulated by rev. Two independent cis-acting elements localized in the gag and env regions are responsible for the low levels of gag and env mRNA in the absence of rev. These elements are different than the rev-responsive element and act independent of each other.

Similar articles

• Elements distinct from human immunodeficiency virus type 1 splice sites are responsible for the Rev dependence of env mRNA.

  Nasioulas G, Zolotukhin AS, Tabernero C, Solomin L, Cunningham CP, Pavlakis GN, Felber BK. Nasioulas G, et al. J Virol. 1994 May;68(5):2986-93. doi: 10.1128/JVI.68.5.2986-2993.1994. J Virol. 1994. PMID: 8151769 Free PMC article.

• Synergistic stimulation of HIV-1 rev-dependent export of unspliced mRNA to the cytoplasm by hnRNP A1.

  Najera I, Krieg M, Karn J. Najera I, et al. J Mol Biol. 1999 Feb 5;285(5):1951-64. doi: 10.1006/jmbi.1998.2473. J Mol Biol. 1999. PMID: 9925777

• Regulation of human immunodeficiency virus env expression by the rev gene product.

  Hammarskjöld ML, Heimer J, Hammarskjöld B, Sangwan I, Albert L, Rekosh D. Hammarskjöld ML, et al. J Virol. 1989 May;63(5):1959-66. doi: 10.1128/JVI.63.5.1959-1966.1989. J Virol. 1989. PMID: 2704072 Free PMC article.

• Regulation of expression of human immunodeficiency virus.

  Pavlakis GN, Felber BK. Pavlakis GN, et al. New Biol. 1990 Jan;2(1):20-31. New Biol. 1990. PMID: 2078551 Review.

• Rev-dependent expression of three species of HIV-1 mRNAs (review).

  Fukumori T, Kagawa S, Iida S, Oshima Y, Akari H, Koyama AH, Adachi A. Fukumori T, et al. Int J Mol Med. 1999 Mar;3(3):297-302. doi: 10.3892/ijmm.3.3.297. Int J Mol Med. 1999. PMID: 10028055 Review.

Cited by

• ADAR1 is a novel multi targeted anti-HIV-1 cellular protein.

  Biswas N, Wang T, Ding M, Tumne A, Chen Y, Wang Q, Gupta P. Biswas N, et al. Virology. 2012 Jan 20;422(2):265-77. doi: 10.1016/j.virol.2011.10.024. Epub 2011 Nov 21. Virology. 2012. PMID: 22104209 Free PMC article.

• Translation of MMTV Gag requires nuclear events involving splicing motifs in addition to the viral Rem protein and RmRE.

  Boeras I, Sakalian M, West JT. Boeras I, et al. Retrovirology. 2012 Jan 25;9:8. doi: 10.1186/1742-4690-9-8. Retrovirology. 2012. PMID: 22277305 Free PMC article.

• The Rev protein of human immunodeficiency virus type 1 promotes polysomal association and translation of gag/pol and vpu/env mRNAs.

  D'Agostino DM, Felber BK, Harrison JE, Pavlakis GN. D'Agostino DM, et al. Mol Cell Biol. 1992 Mar;12(3):1375-86. doi: 10.1128/mcb.12.3.1375-1386.1992. Mol Cell Biol. 1992. PMID: 1545819 Free PMC article.

• Complementation of the rev gene mutation among human and simian lentiviruses.

  Sakai H, Shibata R, Miura T, Hayami M, Ogawa K, Kiyomasu T, Ishimoto A, Adachi A. Sakai H, et al. J Virol. 1990 May;64(5):2202-7. doi: 10.1128/JVI.64.5.2202-2207.1990. J Virol. 1990. PMID: 2182909 Free PMC article.

• Efficient replication of human immunodeficiency virus type 1 requires a threshold level of Rev: potential implications for latency.

  Pomerantz RJ, Seshamma T, Trono D. Pomerantz RJ, et al. J Virol. 1992 Mar;66(3):1809-13. doi: 10.1128/JVI.66.3.1809-1813.1992. J Virol. 1992. PMID: 1738210 Free PMC article.

References

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central

• Other Literature Sources

  • The Lens - Patent Citations Database