Photoactive yellow protein from the purple phototrophic bacterium, Ectothiorhodospira halophila. Quantum yield of photobleaching and effects of temperature, alcohols, glycerol, and sucrose on kinetics of photobleaching and recovery - PubMed
Photoactive yellow protein from the purple phototrophic bacterium, Ectothiorhodospira halophila. Quantum yield of photobleaching and effects of temperature, alcohols, glycerol, and sucrose on kinetics of photobleaching and recovery
T E Meyer et al. Biophys J. 1989 Sep.
Abstract
A water-soluble yellow protein from E. halophila was previously shown to be photoactive (Meyer, T. E., E. Yakali, M. A. Cusanovich, and G. Tollin. 1987. Biochemistry. 26:418-423). Pulsed laser excitation in the protein visible absorption band (maximum at 445 nm) causes a rapid bleach of color (k = 7.5 x 10(3) s-1) followed by a slower dark recovery (k = 2.6 s-1). This is analogous to the photocycle of sensory rhodopsin II from Halobacterium (which also has k = 2.6 s-1 for recovery). We have now determined the quantum yield of the photobleaching process to be 0.64, which is comparable with that of bacteriorhodopsin (0.25), and is thus large enough to be biologically significant. Although the photoreactions of yellow protein were previously shown to be relatively insensitive to pH, ionic strength and the osmoregulator betaine, the present experiments demonstrate that temperature, glycerol, sucrose, and various alcohol-water mixtures strongly influence the kinetics of photobleaching and recovery. The effect of temperature follows normal Arrhenius behavior for the bleach reaction (Ea = 15.5 kcal/mol). The rate constant for the recovery reaction increases with temperature between 5 degrees C and 35 degrees C, but decreases above 35 degrees C indicating alternate conformations with differing kinetics. There is an order of magnitude decrease in the rate constant for photobleaching in both glycerol and sucrose solutions that can be correlated with the changes in viscosity. We conclude from this that the protein undergoes a conformational change as a consequence of the photoinduced bleach. Recovery kinetics are affected by glycerol and sucrose to a much smaller extent and in a more complicated manner. Aliphatic, monofunctional alcohol-water solutions increase the rate constant for the bleach reaction and decrease the rate constant for the recovery reaction, each by an order of magnitude. These effects do not correlate with dielectric constant, indicating that the photocycle probably does not involve separation or recombination of charge accessible to the protein surface. However, the effects on both bleaching and recovery correlate well with the relative hydrophobicity(as measured by partition coefficients in detergent/water mixtures), in the order of increasing effectiveness:methanol < ethanol < iso-propanol <n-propanol < n-butanol. We conclude that the change in conformation of the protein induced by light exposes a hydrophobic site to the solvent. This suggests the possibility that light exerts its effect in vivo by exposing a region of the protein for binding to a hydrophobic receptor site in the cell, perhaps to a protein analogous to the chemotactic transducers in the cytoplasmic membranes of enteric bacteria.
Similar articles
-
Hoff WD, van Stokkum IH, van Ramesdonk HJ, van Brederode ME, Brouwer AM, Fitch JC, Meyer TE, van Grondelle R, Hellingwerf KJ. Hoff WD, et al. Biophys J. 1994 Oct;67(4):1691-705. doi: 10.1016/S0006-3495(94)80643-5. Biophys J. 1994. PMID: 7819501 Free PMC article.
-
Meyer TE, Yakali E, Cusanovich MA, Tollin G. Meyer TE, et al. Biochemistry. 1987 Jan 27;26(2):418-23. doi: 10.1021/bi00376a012. Biochemistry. 1987. PMID: 3828315
-
Kyndt JA, Meyer TE, Cusanovich MA. Kyndt JA, et al. Photochem Photobiol Sci. 2004 Jun;3(6):519-30. doi: 10.1039/b315731h. Epub 2004 Apr 20. Photochem Photobiol Sci. 2004. PMID: 15170480 Review.
Cited by
-
Schneider TD. Schneider TD. Nucleic Acids Res. 2010 Oct;38(18):5995-6006. doi: 10.1093/nar/gkq389. Epub 2010 Jun 18. Nucleic Acids Res. 2010. PMID: 20562221 Free PMC article.
-
Krekic S, Nagy D, Taneva SG, Fábián L, Zimányi L, Dér A. Krekic S, et al. Eur Biophys J. 2019 Jul;48(5):465-473. doi: 10.1007/s00249-019-01353-8. Epub 2019 Mar 23. Eur Biophys J. 2019. PMID: 30905045 Free PMC article.
-
Meyer TE, Tollin G, Causgrove TP, Cheng P, Blankenship RE. Meyer TE, et al. Biophys J. 1991 May;59(5):988-91. doi: 10.1016/S0006-3495(91)82313-X. Biophys J. 1991. PMID: 19431791 Free PMC article.
-
Protein folding thermodynamics applied to the photocycle of the photoactive yellow protein.
Van Brederode ME, Hoff WD, Van Stokkum IH, Groot ML, Hellingwerf KJ. Van Brederode ME, et al. Biophys J. 1996 Jul;71(1):365-80. doi: 10.1016/S0006-3495(96)79234-2. Biophys J. 1996. PMID: 8804619 Free PMC article.
-
Visualizing reaction pathways in photoactive yellow protein from nanoseconds to seconds.
Ihee H, Rajagopal S, Srajer V, Pahl R, Anderson S, Schmidt M, Schotte F, Anfinrud PA, Wulff M, Moffat K. Ihee H, et al. Proc Natl Acad Sci U S A. 2005 May 17;102(20):7145-50. doi: 10.1073/pnas.0409035102. Epub 2005 May 3. Proc Natl Acad Sci U S A. 2005. PMID: 15870207 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources