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Mechanics and regulation of cytokinesis in budding yeast - PubMed

Review

Mechanics and regulation of cytokinesis in budding yeast

Yogini P Bhavsar-Jog et al. Semin Cell Dev Biol. 2017 Jun.

Abstract

Cytokinesis is essential for the survival of all organisms. It requires concerted functions of cell signaling, force production, exocytosis, and extracellular matrix remodeling. Due to the conservation in core components and mechanisms between fungal and animal cells, the budding yeast Saccharomyces cerevisiae has served as an attractive model for studying this fundamental process. In this review, we discuss the mechanics and regulation of distinct events of cytokinesis in budding yeast, including the assembly, constriction, and disassembly of the actomyosin ring, septum formation, abscission, and their spatiotemporal coordination. We also highlight the key concepts and questions that are common to animal and fungal cytokinesis.

Keywords: Actin; Actomyosin ring; Cell separation; Chitin; ELC; Exocytosis; Formin; IQGAP; Mitotic exit; Myosin; RLC; Saccharomyces cerevisiae; Septin; Septum formation.

Copyright © 2016 Elsevier Ltd. All rights reserved.

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Figures

Fig. 1
Fig. 1

Assembly and execution of key cytokinesis events during the cell cycle.

Fig. 2
Fig. 2

Localization and dynamics of core cytokinesis proteins during the cell cycle. Modified from Wloka et al. 2013, which reports protein dynamics revealed by FRAP analysis.

Fig. 3
Fig. 3

Remodeling of the septin cytoskeleton from an hourglass to a double ring at the onset of cytokinesis. (A and B) Electron micrographs showing orientation of septin filaments in the hourglass and double ring. The paired filaments (green) are perpendicular to the single filaments (magenta), and their orientation with respect to the putative myosin-II filaments (purple) is shown. (C) A model of septin hourglass-to-double ring transition. AMR, actomyosin ring. Modified from Ong et al. 2014.

Fig. 4
Fig. 4

The mitotic exit network and its regulation of cytokinesis. (A) The mitotic exit network. SPB, spindle pole body. (B) Spatiotemporal control of AMR constriction and PS formation by MEN and IQGAP. AMR, actomyosin ring; PS, primary septum.

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