Complete gene sequence of spider attachment silk protein (PySp1) reveals novel linker regions and extreme repeat homogenization - PubMed
- ️Invalid Date
Complete gene sequence of spider attachment silk protein (PySp1) reveals novel linker regions and extreme repeat homogenization
Ro Crystal Chaw et al. Insect Biochem Mol Biol. 2017 Feb.
Free article
Abstract
Spiders use a myriad of silk types for daily survival, and each silk type has a unique suite of task-specific mechanical properties. Of all spider silk types, pyriform silk is distinct because it is a combination of a dry protein fiber and wet glue. Pyriform silk fibers are coated with wet cement and extruded into "attachment discs" that adhere silks to each other and to substrates. The mechanical properties of spider silk types are linked to the primary and higher-level structures of spider silk proteins (spidroins). Spidroins are often enormous molecules (>250 kDa) and have a lengthy repetitive region that is flanked by relatively short (∼100 amino acids), non-repetitive amino- and carboxyl-terminal regions. The amino acid sequence motifs in the repetitive region vary greatly between spidroin type, while motif length and number underlie the remarkable mechanical properties of spider silk fibers. Existing knowledge of pyriform spidroins is fragmented, making it difficult to define links between the structure and function of pyriform spidroins. Here, we present the full-length sequence of the gene encoding pyriform spidroin 1 (PySp1) from the silver garden spider Argiope argentata. The predicted protein is similar to previously reported PySp1 sequences but the A. argentata PySp1 has a uniquely long and repetitive "linker", which bridges the amino-terminal and repetitive regions. Predictions of the hydrophobicity and secondary structure of A. argentata PySp1 identify regions important to protein self-assembly. Analysis of the full complement of A. argentata PySp1 repeats reveals extreme intragenic homogenization, and comparison of A. argentata PySp1 repeats with other PySp1 sequences identifies variability in two sub-repetitive expansion regions. Overall, the full-length A. argentata PySp1 sequence provides new evidence for understanding how pyriform spidroins contribute to the properties of pyriform silk fibers.
Keywords: Argiope argentata; Intragenic homogenization; Linker; Pyriform silk.
Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.
Similar articles
-
Analysis of the Full-Length Pyriform Spidroin Gene Sequence.
Wang K, Wen R, Jia Q, Liu X, Xiao J, Meng Q. Wang K, et al. Genes (Basel). 2019 Jun 3;10(6):425. doi: 10.3390/genes10060425. Genes (Basel). 2019. PMID: 31163680 Free PMC article.
-
Intragenic homogenization and multiple copies of prey-wrapping silk genes in Argiope garden spiders.
Chaw RC, Zhao Y, Wei J, Ayoub NA, Allen R, Atrushi K, Hayashi CY. Chaw RC, et al. BMC Evol Biol. 2014 Feb 20;14:31. doi: 10.1186/1471-2148-14-31. BMC Evol Biol. 2014. PMID: 24552485 Free PMC article.
-
Hybrid sequencing reveals the full-length Nephila pilipes pyriform spidroin 1 (PySp1).
Liu FYC, Liu JYX, Yao X, Wang B. Liu FYC, et al. Int J Biol Macromol. 2022 Mar 1;200:362-369. doi: 10.1016/j.ijbiomac.2021.12.078. Epub 2021 Dec 30. Int J Biol Macromol. 2022. PMID: 34973986
-
Molecular mechanisms of spider silk.
Hu X, Vasanthavada K, Kohler K, McNary S, Moore AM, Vierra CA. Hu X, et al. Cell Mol Life Sci. 2006 Sep;63(17):1986-99. doi: 10.1007/s00018-006-6090-y. Cell Mol Life Sci. 2006. PMID: 16819558 Free PMC article. Review.
-
Spider silks from plants - a challenge to create native-sized spidroins.
Hauptmann V, Weichert N, Rakhimova M, Conrad U. Hauptmann V, et al. Biotechnol J. 2013 Oct;8(10):1183-92. doi: 10.1002/biot.201300204. Biotechnol J. 2013. PMID: 24092675 Review.
Cited by
-
Tang X, Ye X, Wang X, Zhao S, Wu M, Ruan J, Zhong B. Tang X, et al. Sci Rep. 2021 Oct 25;11(1):20980. doi: 10.1038/s41598-021-00029-8. Sci Rep. 2021. PMID: 34697320 Free PMC article.
-
A recombinant chimeric spider pyriform-aciniform silk with highly tunable mechanical performance.
Ghimire A, Xu L, Liu XQ, Rainey JK. Ghimire A, et al. Mater Today Bio. 2024 Apr 27;26:101073. doi: 10.1016/j.mtbio.2024.101073. eCollection 2024 Jun. Mater Today Bio. 2024. PMID: 38711935 Free PMC article.
-
Whaite AD, Wang T, Macdonald J, Cummins SF. Whaite AD, et al. PLoS One. 2018 Oct 17;13(10):e0204243. doi: 10.1371/journal.pone.0204243. eCollection 2018. PLoS One. 2018. PMID: 30332416 Free PMC article.
-
Chaw RC, Collin M, Wimmer M, Helmrick KL, Hayashi CY. Chaw RC, et al. G3 (Bethesda). 2018 Jan 4;8(1):231-238. doi: 10.1534/g3.117.300283. G3 (Bethesda). 2018. PMID: 29127108 Free PMC article.
-
Peng X, Liu Z, Gao J, Zhang Y, Wang H, Li C, Lv X, Gao Y, Deng H, Zhao B, Gao T, Li H. Peng X, et al. Molecules. 2024 Feb 27;29(5):1025. doi: 10.3390/molecules29051025. Molecules. 2024. PMID: 38474537 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources