Biomimetic spinning of artificial spider silk from a chimeric minispidroin - PubMed
. 2017 Mar;13(3):262-264.
doi: 10.1038/nchembio.2269. Epub 2017 Jan 9.
Qiupin Jia 2 , Ana Abella 3 , Xiau-Yeen Lee 3 , Michael Landreh 4 , Pasi Purhonen 5 6 , Hans Hebert 5 6 , Maria Tenje 7 8 , Carol V Robinson 4 , Qing Meng 2 , Gustavo R Plaza 3 , Jan Johansson 1 9 , Anna Rising 1 9
Affiliations
- PMID: 28068309
- DOI: 10.1038/nchembio.2269
Biomimetic spinning of artificial spider silk from a chimeric minispidroin
Marlene Andersson et al. Nat Chem Biol. 2017 Mar.
Abstract
Herein we present a chimeric recombinant spider silk protein (spidroin) whose aqueous solubility equals that of native spider silk dope and a spinning device that is based solely on aqueous buffers, shear forces and lowered pH. The process recapitulates the complex molecular mechanisms that dictate native spider silk spinning and is highly efficient; spidroin from one liter of bacterial shake-flask culture is enough to spin a kilometer of the hitherto toughest as-spun artificial spider silk fiber.
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