Saccharomyces cerevisiae Shuttle vectors - PubMed
Review
. 2017 May;34(5):205-221.
doi: 10.1002/yea.3228. Epub 2017 Feb 21.
Affiliations
- PMID: 28072905
- DOI: 10.1002/yea.3228
Review
Saccharomyces cerevisiae Shuttle vectors
Robert Gnügge et al. Yeast. 2017 May.
Abstract
Yeast shuttle vectors are indispensable tools in yeast research. They enable cloning of defined DNA sequences in Escherichia coli and their direct transfer into Saccharomyces cerevisiae cells. There are three types of commonly used yeast shuttle vectors: centromeric plasmids, episomal plasmids and integrating plasmids. In this review, we discuss the different plasmid systems and their characteristic features. We focus on their segregational stability and copy number and indicate how to modify these properties. Copyright © 2017 John Wiley & Sons, Ltd.
Keywords: Saccharomyces cerevisiae; copy number; genomic integration; plasmid loss; plasmids; shuttle vectors.
Copyright © 2017 John Wiley & Sons, Ltd.
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